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Titolo:
DNA content of granulocytes, monocytes, and lymphocytes in the bone marrowsmears of patients with myelodysplastic syndromes
Autore:
Widell, S; Hast, R; Cox, C; Auer, G; Bennett, JM;
Indirizzi:
Karolinska Inst, Karolinska Hosp, Dept Pathol & Oncol, Ctr Canc, S-10401 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-10401 Canc, S-10401 Stockholm, Sweden Karolinska Inst, Karolinska Hosp, Dept Med, Div Hematol, S-10401 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-10401 atol, S-10401 Stockholm, Sweden Univ Rochester, Med Ctr, Dept Biostat, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 pt Biostat, Rochester, NY 14642 USA Univ Rochester, Ctr Canc, Rochester, NY USA Univ Rochester Rochester NY USA v Rochester, Ctr Canc, Rochester, NY USA
Titolo Testata:
AMERICAN JOURNAL OF HEMATOLOGY
fascicolo: 2, volume: 67, anno: 2001,
pagine: 112 - 118
SICI:
0361-8609(200106)67:2<112:DCOGMA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LYMPHOBLASTIC-LEUKEMIA; CELL LINEAGE INVOLVEMENT; IN-SITU HYBRIDIZATION; IMAGE CYTOMETRY; PROGNOSTIC IMPORTANCE; CLONAL ANALYSIS; FLOW-CYTOMETRY; APOPTOSIS; MDS; ANEUPLOIDY;
Keywords:
DNA cytometry; cytogenetics; myelodysplastic syndromes; leukemia; intramarrow cell death;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Widell, S Karolinska Hosp, Dept Pathol & Oncol, Canc Ctr R804, Div Cellular & Mol Tumorpathol, S-10401 Stockholm, Sweden Karolinska Hosp Stockholm Sweden S-10401 401 Stockholm, Sweden
Citazione:
S. Widell et al., "DNA content of granulocytes, monocytes, and lymphocytes in the bone marrowsmears of patients with myelodysplastic syndromes", AM J HEMAT, 67(2), 2001, pp. 112-118

Abstract

Recently, we have reported a high incidence of DNA hypodiploidy defined asDNA index (DI) in blasts/promyelocytes from 39 patients with myelodysplastic syndromes (MDS) found to be without a relationship to cytogenetics, In the present study the DNA content (DI) in granulocytes, monocytes, and lymphocytes measured in the same bone marrow smears from the above patients are reported. DNA hypodiploidy was found in mature cells, not only in myeloid cells (granulocytes and monocytes) but also in lymphocytes, A lower mean DI in each cell type of patients compared to controls was found. Pairwise comparison of the mean DI (+/-SE) in 32 patients with normal (n = 22) and abnormal (n = 10) cytogenetics and controls (n = 8) showed a significantly (P < 0.01) lower value for each group of patients, respectively, in all cell types. No difference was found between the two groups of patients. Presence ofweak-feulgen stained nuclei (DI < 0.40) in granulocytes and monocytes was more pronounced in patients expressing DNA hypodiploid immature cell populations, but only occasionally in lymphocytes, suggesting a link to an apoptotic event and intramedullary cell death. DNA hypodiploidy is shown to be a common feature even in mature cell populations in MDS bone marrows. Clonality, by means of DNA content, appears reasonable as regards the granulocytesand monocytes, DNA hypodiploid lymphocytes, on the other hand, might be small blasts (stem cells) or dying cell populations of unknown origin. Am. J,Hematol, 67:112-118, 2001, (C) 2001 Wiley-Liss, Inc.

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Documento generato il 20/09/20 alle ore 07:32:15