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Titolo:
Comparative sensitivity of human fetal and adult erythrocytes to hemolysisby pulsed 1 MHz ultrasound
Autore:
Miller, MW; Brayman, AA; Sherman, TA; Abramowicz, JS; Cox, C;
Indirizzi:
Univ Rochester, Sch Med & Dent, Dept Obstet & Gynecol, Rochester, NY 14642USA Univ Rochester Rochester NY USA 14642 t & Gynecol, Rochester, NY 14642USA Univ Rochester, Sch Med & Dent, Dept Radiol, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 ept Radiol, Rochester, NY 14642 USA Univ Rochester, Sch Med & Dent, Dept Biostat, Rochester, NY 14642 USA UnivRochester Rochester NY USA 14642 pt Biostat, Rochester, NY 14642 USA
Titolo Testata:
ULTRASOUND IN MEDICINE AND BIOLOGY
fascicolo: 3, volume: 27, anno: 2001,
pagine: 419 - 425
SICI:
0301-5629(200103)27:3<419:CSOHFA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOCRIT HUMAN ERYTHROCYTES; LYSIS IN-VITRO; CELL-LYSIS; 40-PERCENT HEMATOCRIT; LENGTH DEPENDENCE; 1-MHZ ULTRASOUND; EXPOSURE SYSTEM; CONTRAST AGENT; CW ULTRASOUND; CAVITATION;
Keywords:
human; fetal blood; adult blood; sonolysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, MW Univ Rochester, Sch Med & Dent, Dept Obstet & Gynecol, Box 668,Rochester,NY 14642 USA Univ Rochester Box 668 Rochester NY USA 14642 ster,NY 14642 USA
Citazione:
M.W. Miller et al., "Comparative sensitivity of human fetal and adult erythrocytes to hemolysisby pulsed 1 MHz ultrasound", ULTRASOUN M, 27(3), 2001, pp. 419-425

Abstract

Human fetal and adult erythrocytes differ significantly in mean corpuscular volume (MCV), the fetal cells being larger than adult cells and diminishing in MCV as gestational age (GA) increases, Previous studies have shown that the sensitivity of erythrocytes from different species to lysis by mechanically applied shear stress increases as MCV increases. The tested hypotheses in the present project were: 1. fetal erythrocytes would be more sensitive to sonolysis than adult erythrocytes because of the former's larger size, and 2. erythrocyte sonolytic sensitivity would scale with MCV. Fetal andadult erythrocytes were resuspended to 40% hematocrit in oxygenated isotonic saline solution and 500 muL aliquots were exposed for 60 s to 200 mus bursts of 1-MHz ultrasound (US) (peak pressures: similar to4.8 MPa positive, similar to2.7 MPa negative; duty factor = 0.01), either with or without 3.6volume % Albunex (R) (ALS) present, Background-corrected hemolysis was indistinguishable from zero in sham-er;posed fetal or adult erythrocyte suspensions, Without ALX, mean background-corrected US-induced hemolysis was significantly greater than zero for fetal and adult cells (0.42 +/- 0.15% vs. 0.62 +/- 0.15%), but fetal cell lysis was not significantly greater than adult cell lysis, With ALX, US-induced hemolytic yields increased similar to 80-fold (fetal: 50.53 +/- 2.14%; adult: 46.40 +/- 1.85%), and were significantly higher for fetal than for adult cells. There was also a statistically significant correlation between MCV and US-induced background-corrected hemolysis, Thus, the two hypotheses were supported. (C) World Federation for Ultrasound in Medicine & Biology.

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Documento generato il 01/04/20 alle ore 18:26:17