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Titolo:
Gene expression of VEGF and its receptors Flk-1/KDR and Flt-1 in cultured and transplanted rat islets
Autore:
Vasir, B; Jonas, JC; Steil, GM; Hollister-Lock, J; Hasenkamp, W; Sharma, A; Bonner-Weir, S; Weir, GC;
Indirizzi:
Joslin Diabet Ctr, Div Res, Sect Islet Transplantat & Cell Biol, Boston, MA 02215 USA Joslin Diabet Ctr Boston MA USA 02215 t & Cell Biol, Boston, MA 02215 USA Harvard Univ, Sch Med, Dept Med, Boston, MA 02215 USA Harvard Univ BostonMA USA 02215 Sch Med, Dept Med, Boston, MA 02215 USA
Titolo Testata:
TRANSPLANTATION
fascicolo: 7, volume: 71, anno: 2001,
pagine: 924 - 935
SICI:
0041-1337(20010415)71:7<924:GEOVAI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; HYPOXIA-INDUCIBLE FACTOR-1; ORGANIC CATION TRANSPORTER; RENAL MESANGIAL CELLS; UP-REGULATION; PANCREATIC-ISLETS; TYROSINE KINASE; NITRIC-OXIDE; IN-VITRO; KIDNEY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Weir, GC Joslin Diabet Ctr, Div Res, Sect Islet Transplantat & Cell Biol, 1 Joslin Pl, Boston, MA 02215 USA Joslin Diabet Ctr 1 Joslin Pl Boston MA USA 02215 n, MA 02215 USA
Citazione:
B. Vasir et al., "Gene expression of VEGF and its receptors Flk-1/KDR and Flt-1 in cultured and transplanted rat islets", TRANSPLANT, 71(7), 2001, pp. 924-935

Abstract

Background. Vascular endothelial growth factor (VEGF) and its two receptortyrosine kinases, Flk-1/ KDR and Flt-1, may play an important role in mediating the revascularization of transplanted pancreatic islets. Methods. Using semiquantitative multiplex reverse-transcribed polymerase chain reaction we determined the gene expression of VEGF and its receptors in cultured and transplanted rat islets. Results. After exposure of islet cells to hypoxia in vitro, increases werefound in the gene expression of the VEGF120 and VEGF164 isoforms, with simultaneous increases in VE-cadherin, Flk-1/KDR, and Flt-1, In vivo studies consisted of analysis of islet grafts transplanted into both normal and diabetic recipients. Expression of both VEGF120 and VEGF164 in grafts was up-regulated for the first 2-3 days after transplantation, with the response being more prolonged in the diabetic rats. These increases were followed by reduced expression of VEGF on days 5, 7, and 9. Increases in the expression of VE-cadherin in islet grafts in normal and diabetic recipients tended to parallel VEGF expression, with the increases in both probably being caused by hypoxia, The early increases of VE:GF expression were followed by a rise in the expression of VEGF receptors, which probably represents the early stages of angiogenesis. Graft expression of Flk-1/KDR and Flt-1 was enhanced at 3 and 5 days in the normoglycemic recipients, while in the diabetic recipients increases were found later on days 5, 7, and 14. Conclusions. The delayed expression of VEGF receptors in the diabetic recipients could reflect impaired angiogenesis caused by the diabetic milieu; this delay could contribute to the less outcomes of grafts transplanted intoa hyperglycemic environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:35:15