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Titolo:
Effect of MPTP-induced denervation on basal ganglia GABA(B) receptors: Correlation with dopamine concentrations and dopamine transporter
Autore:
Calon, F; Lavertu, N; Lemieux, AM; Morissette, M; Goulet, M; Grondin, R; Blanchet, PJ; Bedard, PJ; Di Paolo, T;
Indirizzi:
Univ Laval, Med Ctr, CHUL, Oncol & Mol Endocrinol Res Ctr, St Foy, PQ G1V 4G2, Canada Univ Laval St Foy PQ Canada G1V 4G2 l Res Ctr, St Foy, PQ G1V 4G2, Canada Univ Laval, Fac Pharm, St Foy, PQ G1V 4G2, Canada Univ Laval St Foy PQ Canada G1V 4G2 Fac Pharm, St Foy, PQ G1V 4G2, Canada Univ Laval, Med Ctr, CHUL, Neurosci Res Unit, St Foy, PQ G1V 4G2, Canada Univ Laval St Foy PQ Canada G1V 4G2 Res Unit, St Foy, PQ G1V 4G2, Canada Univ Laval, Fac Med, Dept Med, St Foy, PQ G1V 4G2, Canada Univ Laval St Foy PQ Canada G1V 4G2 Dept Med, St Foy, PQ G1V 4G2, Canada
Titolo Testata:
SYNAPSE
fascicolo: 3, volume: 40, anno: 2001,
pagine: 225 - 234
SICI:
0887-4476(20010601)40:3<225:EOMDOB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; IN-SITU HYBRIDIZATION; RAT-BRAIN; MESSENGER-RNA; SPLICE VARIANTS; BINDING-SITES; LOCALIZATION; EXPRESSION; MONKEYS; MICE;
Keywords:
substantia nigra; Parkinson; dopamine; GABA; autoradiography; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Di Paolo, T Univ Laval, Med Ctr, CHUL, Oncol & Mol Endocrinol Res Ctr, 2705 Laurier Blvd, St Foy, PQ G1V 4G2, Canada Univ Laval 2705 Laurier Blvd St Foy PQ Canada G1V 4G2 , Canada
Citazione:
F. Calon et al., "Effect of MPTP-induced denervation on basal ganglia GABA(B) receptors: Correlation with dopamine concentrations and dopamine transporter", SYNAPSE, 40(3), 2001, pp. 225-234

Abstract

We investigated the effect of MPTP-induced lesion of the substantia nigra pars compacta (SNpc) dopaminergic neurons on GABA(B) receptors in the basalganglia of mice and monkeys using receptor autoradiography and in situ hybridization. The extent of the lesion was measured with striatal catecholamine content, striatal binding of I-125-RTI-121 to dopamine transporter (DAT), and DAT expression in the SNpc. GABA(B) receptors in mice brain were evaluated using H-3-CGP54626 and its expression was measured with oligonucleotides probes targeting the mRNAs of GABA(B(1a + b)), GABA(B(1a)), GABA(B(1b)), GABA(B(2)) subunits. In monkeys, I-125-CGP64213 and selective probes for GABA(B(1a + b)) and GABA(B(2)) mRNAs were used. In mice, dopamine content, I-125-RTI-121 binding, and DAT expression were reduced by 44%, 40%, and 39%after a dose of 40 mg/kg of MPTP and 74%, 70%, and 34% after 120 mg/kg of MPTP, respectively. In monkeys, dopamine content and DAT expression were decreased by more than 90% and 80%, respectively. In the striatum and the subthalamic nucleus, GABA(B) receptors were unchanged following MPTP in both species. In the SNpc of mice, MPTP (120 mg/kg) induced a significant decrease of H-3-CGP54626 binding (-10%) and of the expression of GABA(B(1a + b)) mRNA (-13%). The decrease of the expression of GABA(B(1a + b)) mRNA was correlated with dopamine content, I-125-RTI-121 binding and DAT expression. In MPTP-treated monkeys, I-125-CGP64213 binding (-40%), GABA(B(1a + b)) mRNA (-69%) and GABA(B(2)), mRNA (-66%) were also significantly decreased in the SNpc. Our results suggest that MPTP-induced denervation is associated with a decrease of GABA(B) receptors restricted to the SNpc. These observations may be relevant to the pathophysiology of motor disorders involving dysfunction of the basal ganglia such as Parkinson disease. Synapse 40:225-234, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:52:48