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Titolo:
Construction and immunogenicity prediction of Plasmodium falciparum CTL epitope minigene vaccine
Autore:
Tang, YY; Wang, H;
Indirizzi:
Chinese Acad Med Sci, Sch Bas Med, Inst Basic Med Sci, Peking Union Med Coll, Beijing 100005, Peoples R China Chinese Acad Med Sci Beijing Peoples R China 100005 005, Peoples R China
Titolo Testata:
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
fascicolo: 2, volume: 44, anno: 2001,
pagine: 207 - 215
SICI:
1006-9305(200104)44:2<207:CAIPOP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL EPITOPES; PEPTIDE; HLA; MALARIA; BINDING;
Keywords:
Plasmodium falciparum; CD8(+) cytotoxic T cell; MHC class I molecule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, H Chinese Acad Med Sci, Sch Bas Med, Inst Basic Med Sci, Peking Union Med Coll, Beijing 100005, Peoples R China Chinese Acad Med Sci Beijing Peoples R China 100005 ples R China
Citazione:
Y.Y. Tang e H. Wang, "Construction and immunogenicity prediction of Plasmodium falciparum CTL epitope minigene vaccine", SCI CHINA C, 44(2), 2001, pp. 207-215

Abstract

The minigenes encoding Plasmodium falciparum CTL epitopes restricted to human MHC class I molecular HLA-A2 and HLA-B51, which were both at high frequency among Chinese population, were constructed as mono-epitope CTL vaccines named pcDNA3.1/tr and pcDNA3.1/sh. The minigenes of the two epitopes werethen tandem linked to form a dimeric CTL epitope minigene recombinant vaccine. After DNA transfection, the epitope minigenes were expressed respectively in two human cell lines, each bearing one MHC class I molecule named CIR/HLA-A2.1 and K562/HLA-B51. The intracellular expression of the CTL epitope minigenes not only enhanced the stability of HLA-A2.1 and HLA-B51 molecules but also increased the assemblage of MHC class I molecules on cell surfaces, which testified the specific process and presentation of those endogenous expressed epitopes. For the cells transfected with the dimeric minigeneencoding two tandem linked epitopes, the expression and presentation of each epitope were also detected on cell membranes that bore different MHC class I molecules. It meant that the adjacency of the two CTL epitopes did notinterfere with the specific process and presentation of each epitope. Compared with the ordinary CTL studies that inoculated synthesized epitope peptides with peripheral blood cells, this work aimed to process the epitopes directly inside HLA class I allele specific human cells, and thus theoretically imitated the same procedure in vivo. It was also an economical way to predict the immunogenicity of CTL epitopes at an early stage especially in laboratories with limited financial resource.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:00:10