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Titolo:
Maize endosperm secretes a novel antifungal protein into adjacent maternaltissue
Autore:
Serna, A; Maitz, M; OConnell, T; Santandrea, G; Thevissen, K; Tienens, K; Hueros, G; Faleri, C; Cai, G; Lottspeich, F; Thompson, RD;
Indirizzi:
MPI Zuchtungsforsch, D-50829 Cologne, Germany MPI Zuchtungsforsch Cologne Germany D-50829 ch, D-50829 Cologne, Germany Katholieke Univ Leuven, Louvain, Belgium Katholieke Univ Leuven Louvain Belgium ke Univ Leuven, Louvain, Belgium Univ Siena, Dept Environm Sci, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 , Dept Environm Sci, I-53100 Siena, Italy MPI Biochem, Martinsried, Germany MPI Biochem Martinsried GermanyMPI Biochem, Martinsried, Germany
Titolo Testata:
PLANT JOURNAL
fascicolo: 6, volume: 25, anno: 2001,
pagine: 687 - 698
SICI:
0960-7412(200103)25:6<687:MESANA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIMICROBIAL PEPTIDES; PRECURSOR PROTEIN; PLANT DEFENSINS; FUNGAL GROWTH; SEEDS; PURIFICATION; EXPRESSION; MICROSCOPY; KERNELS; FAMILY;
Keywords:
endosperm; transfer layer; antifungal; defensin; protease; transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Thompson, RD MPI Zuchtungsforsch, Carl von Linne Weg 10, D-50829 Cologne, Germany MPI Zuchtungsforsch Carl von Linne Weg 10 Cologne Germany D-50829
Citazione:
A. Serna et al., "Maize endosperm secretes a novel antifungal protein into adjacent maternaltissue", PLANT J, 25(6), 2001, pp. 687-698

Abstract

A series of endosperm transfer layer-specific transcripts has been identified in maize by differential screening of a cDNA library of transcripts at 10 days after pollination. Sequence comparisons revealed among this class of cDNAs a novel, small gene family of highly diverged sequences encoding basal layer antifungal proteins (BAPs). The bap genes mapped to two loci on chromosomes 4 and 10. So far, bap-homologous sequences have been detected only in maize, teosinte and sorghum, and are not present in grasses outside the Andropogoneae tribe. BAP2 is synthesized as a pre-proprotein, and is processed by successive removal of a signal peptide and a 29-residue prodomain. The proprotein can be detected exclusively in microsomal membrane-containing fractions of kernel extracts. Immunolocalization reveals BAP2 to be predominantly located in the placentochalazal cells of the pedicel, adjacent to the basal endosperm transfer layer (BETL) cells, although the BAPS transcript is found only in the BETL cells. The biological roles of BAP2 propeptide and mature peptide have been investigated by heterologous expression of the proprotein in Escherichia coil, and by tests of its fungistatic activity and that of the fully processed form in vitro. The mature BAP2 peptide exhibits potent broad-range activity against a range of filamentous fungi, including several plant pathogens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 05:11:58