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Titolo:
Kinetics of coaggregation of Porphyromonas gingivalis with Fusobacterium nucleatum using an automated microtiter plate assay
Autore:
Metzger, Z; Featherstone, LG; Ambrose, WW; Trope, M; Arnold, RR;
Indirizzi:
Univ N Carolina, Sch Dent, Dept Endodont, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA t, Dept Endodont, Chapel Hill, NC USA Univ N Carolina, Sch Dent, Dent Res Ctr, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA nt, Dent Res Ctr, Chapel Hill, NC USA Univ N Carolina, Sch Dent, Dept Periodont & Diagnost Sci, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA t & Diagnost Sci, Chapel Hill, NC USA
Titolo Testata:
ORAL MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 3, volume: 16, anno: 2001,
pagine: 163 - 169
SICI:
0902-0055(200106)16:3<163:KOCOPG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSE-BINDING ADHESIN; BACTEROIDES-GINGIVALIS; MOUSE MODEL; BACTERIA; STRAINS; PK1594; T22; T18;
Keywords:
coaggregation; Fusobacterium nucleatum; Porphyromonas gingivalis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Metzger, Z Tel Aviv Univ, Maurice & Gabriela Goldschleger Sch Dent Med, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 69978 Tel Aviv, Israel
Citazione:
Z. Metzger et al., "Kinetics of coaggregation of Porphyromonas gingivalis with Fusobacterium nucleatum using an automated microtiter plate assay", ORAL MICROB, 16(3), 2001, pp. 163-169

Abstract

Coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatumstrains was previously studied using either a semi-quantitative macroscopic assay or radioactive tracer assays. A new automated microtiter plate assay is introduced, in which the plate reader (Vmax) was adapted to allow quantitative evaluation of the kinetics of coaggregation. F nucleatum PK 1594 coaggregated with P. gingivalis HG 405 with a maximal coaggregation rate of 1.05 mOD/min, which occurred at a P. gingivalis to F. nucleatum cell ratio of 1 to 2. F. nucleatum PK 1594 failed to do so with P. gingivalis strains A 7436 or ATCC 33277. Galactose inhibition of this coaggregation could be quantitatively measured over a wide range of concentrations to demonstrate its dose-dependent manner. P. gingivalis HG 405 failed to coaggregate with F. nucleatum strains ATCC 25586 and ATCC 49256. The assay used in the present study is a sensitive and efficient quantitative automated tool to study coaggregation and may replace tedious radioactive tracer assays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 19:20:10