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Titolo:
Environmental regulation of recA gene expression in Porphyromonas gingivalis
Autore:
Liu, Y; Fletcher, HM;
Indirizzi:
Loma Linda Univ, Sch Med, Dept Microbiol & Mol Genet, Loma Linda, CA 92350USA Loma Linda Univ Loma Linda CA USA 92350 ol Genet, Loma Linda, CA 92350USA
Titolo Testata:
ORAL MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 3, volume: 16, anno: 2001,
pagine: 136 - 143
SICI:
0902-0055(200106)16:3<136:ERORGE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; CYSTEINE PROTEINASE; BACTEROIDES-FRAGILIS; NUCLEOTIDE-SEQUENCE; EPITHELIAL-CELLS; CALCIUM; W83; INVOLVEMENT; VIRULENCE; GINGIPAIN;
Keywords:
Porphyromonas gingivalis; gene expression; environmental; regulation; mutant; recA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Fletcher, HM Loma Linda Univ, Sch Med, Dept Microbiol & Mol Genet, Loma Linda, CA 92350USA Loma Linda Univ Loma Linda CA USA 92350 a Linda, CA 92350USA
Citazione:
Y. Liu e H.M. Fletcher, "Environmental regulation of recA gene expression in Porphyromonas gingivalis", ORAL MICROB, 16(3), 2001, pp. 136-143

Abstract

The recA gene product in Porphyromonas gingivalis is involved in DNA repair. Further, disruption of this gene can affect the proteolytic activity andexpression of other virulence factors in this organism. Since several known environmental factors can influence virulence gene expression in P. gingivalis, we investigated the influence of these signals on the expression of the recA gene in this organism. A heterodiploid strain of P. gingivalis (designated FLL 118) containing a transcriptional fusion of the recA promoter region and the promoterless tetracycline-resistant gene [tetA (Q)2] and xylosidase/arabinosidase (xa) gene cassette was constructed. The recA promoteractivity was assessed by measurement of xylosidase activity in FLL118. Theexpression remained relatively constant during different growth phases, atdifferent pH levels and in the presence of DNA-damaging agents. In response to hemin limitation and in the presence of calcium there was a moderate increase in recA promoter activity. Temperature also affected the expression. The highest level of xylosidase activity was observed in cultures at 32 degreesC with a decline of approximately 46% as growth temperature increasedto 41 degreesC. Reverse transcriptase polymerase chain reaction analysis revealed that this regulation may be occurring at the transcriptional level. These results suggest that expression of the recA gene in P. gingivalis W83 is responsive to several environmental signals but is not regulated by a DNA damage-inducible SOS-like regulatory system.

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Documento generato il 05/12/20 alle ore 14:10:59