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Titolo:
Expression and characterization of a recombinant Fab fragment derived froman anti-human alpha-fetoprotein monoclonal antibody
Autore:
Kim, SH; Song, SH; Kim, YJ; Park, SY;
Indirizzi:
Korea Green Cross Corp, Cent Res Ctr, Antibody Engn Lab, Yongin 449903, South Korea Korea Green Cross Corp Yongin South Korea 449903 gin 449903, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 2, volume: 11, anno: 2001,
pagine: 158 - 163
SICI:
1016-8478(20010430)11:2<158:EACOAR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL HEPATOCELLULAR-CARCINOMA; PHAGE DISPLAY TECHNOLOGY; SERUM-ALBUMIN; SEQUENCE HOMOLOGY; HIGH-AFFINITY; BINDING; TUMORS; RADIOIMMUNODETECTION; ARACHIDONATE; LIBRARIES;
Keywords:
AFP; bacterial Fab expression; monoclonal antibody; recombinant Fab;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SH Korea Green Cross Corp, Cent Res Ctr, Antibody Engn Lab, Yongin 449903, South Korea Korea Green Cross Corp Yongin South Korea 449903 03, South Korea
Citazione:
S.H. Kim et al., "Expression and characterization of a recombinant Fab fragment derived froman anti-human alpha-fetoprotein monoclonal antibody", MOL CELLS, 11(2), 2001, pp. 158-163

Abstract

Alpha-fetoprotein (AFP) is a well-known molecular marker indicating the development of cancer as well as fetal abnormalities such as open neural tubedefects. Accordingly the measurement of serum AFP is important for the diagnosis of hepatocellular carcinoma (HCC) and other abnormalities. Monoclonal antibodies (McAb) to AFP were produced to develop an immunoassay kit, andto study the possibility of an antibody (Ab) therapy. The immunoglobulin genes were cloned from hybridoma cells, and expressed in E. coli as an Fab soluble into a culture medium. The Fab of anti-AFP McAb exhibited binding toAFP and similar affinity compared to the original Igc, This recombinant antibody can be studied further for in vivo imaging and immunotherapeutics.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:19:43