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Titolo:
Comparative analysis of ras proto-oncogene mutations in selected mammaliantumors
Autore:
Watzinger, F; Mayr, B; Gamerith, R; Vetter, C; Lion, T;
Indirizzi:
St Anna Kiderspital, Childrens Canc Res Inst, A-1090 Vienna, Austria St Anna Kiderspital Vienna Austria A-1090 s Inst, A-1090 Vienna, Austria Vet Univ Vienna, Inst Anim Breeding & Genet, A-1030 Vienna, Austria Vet Univ Vienna Vienna Austria A-1030 ng & Genet, A-1030 Vienna, Austria
Titolo Testata:
MOLECULAR CARCINOGENESIS
fascicolo: 4, volume: 30, anno: 2001,
pagine: 190 - 198
SICI:
0899-1987(200104)30:4<190:CAORPM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL LUNG-CANCER; MALIGNANT FIBROUS HISTIOCYTOMAS; ACUTE MYELOID-LEUKEMIA; SOFT-TISSUE SARCOMA; HUMAN-BREAST CANCER; N-RAS; GENE-MUTATIONS; POINT MUTATIONS; H-RAS; NEURO-BLASTOMA;
Keywords:
canine; feline; and human single-strand conformation polymorphisms; mutation analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Lion, T St Anna Kiderspital, Childrens Canc Res Inst, Kinderspialgasse 6, A-1090 Vienna, Austria St Anna Kiderspital Kinderspialgasse 6 Vienna Austria A-1090 tria
Citazione:
F. Watzinger et al., "Comparative analysis of ras proto-oncogene mutations in selected mammaliantumors", MOL CARCINO, 30(4), 2001, pp. 190-198

Abstract

Point mutations within ras proto-oncogenes are frequently detected in human malignancies and in different types of experimentally induced tumors in animals. In contrast to findings in experimental animal models of carcinogenesis, little is known about the incidence of ras mutations in naturally occurring animal tumors. In the present study, we investigated whether point mutations, particularly within the mutational her-spot codons 12, 13, and 61, occur at comparable frequencies in human malignancies and spontaneously occurring tumors in other mammalian species. Two hundred seventy-nine of themost frequent canine and feline neoplasms were analyzed for changes in mutational hot-spot regions of the N-, Ki-, and Ha-ras genes. DNA fragments from exons 1 and 2 of all three ras genes were amplified by polymerase chain reaction, and the presence of point mutations was assessed by single-strandconformation polymorphism analysis and direct sequencing of amplified products. Only one sample, a case of canine melanoma, exhibited an Ha-ras mutation. Thus, our data strongly suggested that ras mutations at the hot-spot loci are apparently very rare and do not play a major role in the pathogenesis of the spontaneously occurring canine and feline tumors investigated. These observations were in marked contrast to those in experimental rodent models of carcinogen-induced mammary and skin tumors that described a consisrenr association with Ha- or Ki-ras arrivarion. The role of ras oncogene activation in related human malignancies therefore cannot be readily inferred from studies of experimental carcinogenesis in animal models. (C) 2000 Wiley-Liss Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:55:46