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Titolo:
Enhanced activity and level of protein kinase A in the spinal cord supernatant of diisopropyl phosphorofluoridate (DFP)-treated hens. Distribution ofprotein kinases and phosphatases in spinal cord subcellular fractions
Autore:
Gupta, RP; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27708 USA Duke Univ Durham NC USA 27708 Pharmacol & Canc Biol, Durham, NC 27708 USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 220, anno: 2001,
pagine: 15 - 23
SICI:
0300-8177(200104)220:1-2<15:EAALOP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED DELAYED NEUROTOXICITY; MESSENGER-RNA EXPRESSION; PHOSPHORYLATION SITE; CALMODULIN-BINDING; CATALYTIC SUBUNIT; CAMP; BRAIN; RECEPTOR; AREA; PKA;
Keywords:
diisopropyl phosphorofluoridate; chicken; spinal cord; cerebrum; OPIDN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27708USA Duke Univ POB 3813 Durham NC USA 27708 Durham, NC 27708USA
Citazione:
R.P. Gupta e M.B. Abou-Donia, "Enhanced activity and level of protein kinase A in the spinal cord supernatant of diisopropyl phosphorofluoridate (DFP)-treated hens. Distribution ofprotein kinases and phosphatases in spinal cord subcellular fractions", MOL C BIOCH, 220(1-2), 2001, pp. 15-23

Abstract

Diisopropyl phosphorofluoridate (DFP) is a type I organophosphorus compound and produces delayed neurotoxicity (OPIDN) in adult hens. A single dose of DFP (1.7 mg/kg, sc.) produces mild ataxia in hens in 7-14 days, which develops into severe ataxia or paralysis as the disease progresses. We have previously shown altered expression of several proteins (e.g. Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) alpha -subunit, tau, tubulin, neurofilament protein (NF), vimentin, GFAP) and an immediate early gene (e.g. c-fos) in DFP-treated hens. Here we show an increase in protein kinase A(PKA) protein level and activity in the spinal cord at 1-day and 5-days time periods after DFP administration. We also determined the protein levels of protein kinase C (PKC), CaM kinase II and several phosphatases (i.e. phosphatase 1 (PP1), phosphatase 2A (PP2A), phosphatase 2B (PP2B) in the spinal cord of DFP-treated hens after 1, 5, 10, and 20 days). There was increasein CaM kinase II alpha subunit level after 10 and 20 days of treatment, and decrease in PKC level at 1-day and 20-days time periods in spinal cord mitochondria. In contrast, the cerebrum, which is resistant to DFP-induced axonal degeneration, did not show change in PKA and CaM Kinase II levels at any time period DFP post-administration. No alteration was found in the protein levels of PP1, PP2A, and PP2B at any time period. An early induction inPKA, which is an important protein kinase in signal transduction, followedby that of CaM kinase might be contributing towards the development of OPIDN in DFP-treated hens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:58:50