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Titolo:
Endotoxic properties of lipid A from Comamonas testosteroni
Autore:
Tanamoto, K; Iida, T; Haishima, Y; Azumi, S;
Indirizzi:
Natl Inst Hlth Sci, Div Microbiol, Setagaya Ku, Tokyo 1588501, Japan Natl Inst Hlth Sci Tokyo Japan 1588501 Setagaya Ku, Tokyo 1588501, Japan
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 5
pagine: 1087 - 1094
SICI:
1350-0872(200105)147:<1087:EPOLAF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE; RHODOPSEUDOMONAS-SPHAEROIDES; NONTOXIC LIPOPOLYSACCHARIDE; HUMAN MONOCYTES; C3H/HEJ MICE; A COMPONENT; ANTAGONIST; INDUCTION; ANALOGS; LINE;
Keywords:
lipopolysaccharide; LPS; biological activity of lipid A; endotoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Tanamoto, K Natl Inst Hlth Sci, Div Microbiol, Setagaya Ku, 1-18-1 Kamiyoga, Tokyo 1588501, Japan Natl Inst Hlth Sci 1-18-1 Kamiyoga Tokyo Japan 1588501 Japan
Citazione:
K. Tanamoto et al., "Endotoxic properties of lipid A from Comamonas testosteroni", MICROBI-SGM, 147, 2001, pp. 1087-1094

Abstract

The lipid A from comamonas testosteroni has been isolated and its completechemical structure determined [Iida, T., Haishima, Y., Tanaka, A., Nishijima, K., Saito, S. & Tanamoto, K. (1996). Eur J Biochem 237, 468-475]. In this work, the relationship between its chemical structure and biological activity was studied. The lipid A was highly homogeneous chemically and was characterized by the relatively short chain length (C-10) of the 3-hydroxy fatty acid components directly bound to the glucosamine disaccharide backboneby either amide or ester linkages. The lipid A exhibited endotoxic activity in all of the assay systems tested (mitogenicity in mouse spleen cells; induction of tumour necrosis factor alpha release from both mouse peritonealmacrophages and mouse macrophage-like cell line J774-1, as well as from the human monocytic cell line THP-1; induction of nitric oxide release from J774-1 cells; Limulus gelation activity and lethal toxicity in galactosamine-sensitized mice) to the same extent as did 'Salmonella minnesota' lipid A or Escherichia coil LPS used as controls. The strong endotoxic activity of the C. testosteroni lipid A indicates that the composition of 3-hydroxydecanoic acid is not responsible for the low endotoxicity of the lipid A observed in members of the genus Rhodopseudomonas, as has previously been suggested. Furthermore, both the lack of a second acylation of the 3-hydroxy fattyacid attached at the 3' position, and the substitution of the hydroxyl group of the 3-hydroxy fatty acid attached at position 2, do not affect the manifestation of endotoxic activity or species specificity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 09:09:06