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Titolo:
Highly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi
Autore:
Arnold, DL; Jackson, RW; Fillingham, AJ; Goss, SC; Taylor, JD; Mansfield, JW; Vivian, A;
Indirizzi:
Univ W England, Fac Sci Appl, Ctr Res Plant Sci, Bristol BS16 1QY, Avon, England Univ W England Bristol Avon England BS16 1QY stol BS16 1QY, Avon, England Imperial Coll, Dept Biol Sci, Ashford TN25 5AH, Kent, England Imperial Coll Ashford Kent England TN25 5AH hford TN25 5AH, Kent, England Hort Res Int, Warwick CV35 9EF, England Hort Res Int Warwick England CV35 9EF Res Int, Warwick CV35 9EF, England
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 5
pagine: 1171 - 1182
SICI:
1350-0872(200105)147:<1171:HCSFAG>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTIVAR-SPECIFIC AVIRULENCE; DISEASE RESISTANCE GENE; RANGE CLONING VECTOR; MOLECULAR CHARACTERIZATION; PHYTOPATHOGENIC BACTERIA; PATHOGENICITY ISLAND; SATIVUM CULTIVARS; ESCHERICHIA-COLI; PHASEOLICOLA; PEA;
Keywords:
Pseudomonas syringae; conserved DNA sequences; avr; plasmid; rulAB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Arnold, DL Univ W England, Fac Sci Appl, Ctr Res Plant Sci, Coldharbour Lane, BristolBS16 1QY, Avon, England Univ W England Coldharbour Lane Bristol Avon England BS16 1QY d
Citazione:
D.L. Arnold et al., "Highly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi", MICROBI-SGM, 147, 2001, pp. 1171-1182

Abstract

DNA sequences flanking two avr genes (avrPpiA1 and avrPpiB1) from Pseudomonas syringae pv. pisi show a high degree of similarity. Specific primers designed from the conserved regions were used in PCR amplifications with ail P. syringae pv. pisi races. As well as amplifying the expected avrPpiA- andavrPpiB-containing fragments, two additional fragments were amplified: onecontained a single open reading frame (ORF1) and was found in races of genomic group II (2, 3A, 4A and 6); the second fragment contained two open reading frames (ORF2 and ORF3), separated by 658 nt, and was detected in all races. All three ORFs had G+C ratios (46.9-48 mol%) that were significantly less than that for P, syringae and each was preceded by a potential hrp boxpromoter. In P. syringae pv. phaseolicola, ORF1 and ORF2 each elicited a strong non-host hypersensitive reaction on bean leaves; ORF1 was designated avrPpiG, the product of which had strong similarity to AvrRxv, AvrBsT and YopP, ORF2 was identical to a gene, designated avrPpiC, previously isolated from P, syringae pv. pisi race 5. ORF3 was always found in association withavrPpiC and both were detected in a wide range of P, syringae pathovars. In contrast, avrPpiG was only detected in strains of P, syringae pv. pisi genomic group II and P. syringae pv, coronafaciens (ICMP 3113). In P. syringae pv. pisi, avrPpiG was plasmid-borne and avrPpiC and ORF3 were chromosomal. This conservation of flanking sequences has implications for the horizontal transfer of avirulence and virulence genes, suggesting that specific regions of the bacterial genome act as sites for their integration/excision.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 04:21:20