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Titolo:
Structure of the cell envelope of corynebacteria: importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane
Autore:
Puech, V; Chami, M; Lemassu, A; Laneelle, MA; Schiffler, B; Gounon, P; Bayan, N; Benz, R; Daffe, M;
Indirizzi:
Univ Toulouse 3, Inst Pharmacol & Biol Struct, CNRS, UMR 5089, F-31077 Toulouse 04, France Univ Toulouse 3 Toulouse France 04 UMR 5089, F-31077 Toulouse 04, France Univ Paris 11, Lab Biomembranes, CNRS, UMR 8619, F-91405 Orsay, France Univ Paris 11 Orsay France F-91405 CNRS, UMR 8619, F-91405 Orsay, France Univ Wurzburg, Biozentrum, Lehrstuhl Biotechnol, D-97074 Wurzburg, GermanyUniv Wurzburg Wurzburg Germany D-97074 echnol, D-97074 Wurzburg, Germany Inst Pasteur, Serv Microscopie Elect, F-75724 Paris 15, France Inst Pasteur Paris France 15 Microscopie Elect, F-75724 Paris 15, France CNRS, Ctr Genet Mol, F-91190 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91190 t Mol, F-91190 Gif Sur Yvette, France
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 5
pagine: 1365 - 1382
SICI:
1350-0872(200105)147:<1365:SOTCEO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYCOBACTERIUM-BOVIS BCG; CORYNEFORM BACTERIA; MYCOLIC ACIDS; BIOPHYSICAL CHARACTERIZATION; ELECTRON-MICROSCOPY; NUCLEOTIDE-SEQUENCE; FREEZE-FRACTURE; SURFACE-LAYER; TUBERCULOSIS; GLUTAMICUM;
Keywords:
cell wall; corynebacteria; mycolic acid; polysaccharide; porin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Daffe, M Univ Toulouse 3, Inst Pharmacol & Biol Struct, CNRS, UMR 5089, 205 Route Narbonne, F-31077 Toulouse 04, France Univ Toulouse 3 205 Route Narbonne Toulouse France 04 04, France
Citazione:
V. Puech et al., "Structure of the cell envelope of corynebacteria: importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane", MICROBI-SGM, 147, 2001, pp. 1365-1382

Abstract

With the recent success of the heterologous expression of mycobacterial antigens in corynebacteria, in addition to the importance of these bacteria in biotechnology and medicine, a better understanding of the structure of their cell envelopes was needed. A combination of molecular compositional analysis, ultrastructural appearance and freeze-etch electron microscopy studywas used to arrive at a chemical model, unique to corynebacteria but consistent with their phylogenetic relatedness to mycobacteria and other membersof the distinctive suprageneric actinomycete taxon. Transmission electron microscopy and chemical analyses showed that the cell envelopes of the representative strains of corynebacteria examined consisted of (i) an outer layer composed of polysaccharides (primarily a high-molecular-mass glucan and arabinomannans), proteins, which include the mycoloyltransferase PS1, and lipids; (ii) a cell wall glycan core of peptidoglycan-arabinogalactan which may contain other sugar residues and was usually esterified by corynomycolic acids; and (iii) a typical plasma membrane bilayer. Freeze-etch electron microscopy showed that most corynomycolate-containing strains exhibited a main fracture plane in their cell wall and contained low-molecular-mass porins, while the fracture occurred within the plasma membrane of strains devoid of both corynomycolate and pore-forming proteins. Importantly, in most strains, the amount of cell wall-linked corynomycolates was not sufficient tocover the bacterial surface; interestingly, the occurrence of a cell wall fracture plane correlated with the amount of non-covalently bound lipids ofthe strains. Furthermore, these lipids were shown to spontaneously form liposomes, indicating that they may participate in a bilayer structure. Altogether, the data suggested that the cell wall permeability barrier in corynebacteria involved both covalently linked corynomycolates and non-covalentlybound lipids of their cell envelopes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:29:56