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Titolo:
Fast separation of terbinafine and eight of its metabolites by capillary electrophoresis
Autore:
Crego, AL; Gomez, J; Lavandera, JL;
Indirizzi:
Univ Alcala, Fac Quim, Dept Quim Analit, Madrid 28871, Spain Univ Alcala Madrid Spain 28871 im, Dept Quim Analit, Madrid 28871, Spain GlaxoWellcome, Ctr Invest & Desarrollo, Dept Quim Estructural, Madrid 28760, Spain GlaxoWellcome Madrid Spain 28760 t Quim Estructural, Madrid 28760, Spain
Titolo Testata:
JOURNAL OF SEPARATION SCIENCE
fascicolo: 4, volume: 24, anno: 2001,
pagine: 265 - 270
SICI:
1615-9314(200104)24:4<265:FSOTAE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN PLASMA; LAMISIL;
Keywords:
terbinafine; drug metabolites; in-vitro analysis; capillary zone electrophoresis; diode array detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Crego, AL Univ Alcala, Fac Quim, Dept Quim Analit, Madrid 28871, Spain Univ Alcala Madrid Spain 28871 uim Analit, Madrid 28871, Spain
Citazione:
A.L. Crego et al., "Fast separation of terbinafine and eight of its metabolites by capillary electrophoresis", J SEP SCI, 24(4), 2001, pp. 265-270

Abstract

A rapid and simple capillary zone electrophoresis (CZE) method for the determination of terbinafine and eight of its main metabolites after incubation with rat hepatic S9 fraction was developed. The effect of the concentration and type of the running electrolyte as well as of the addition of an organic solvent were studied with emphasis on selectivity and sensitivity. Allnine components obtained (unmetabolized terbinafine and up to eight of itsmetabolites) are baseline resolved in less than 4 minutes using a 0.05 M phosphate buffer (pH 2.2) without additives, after a solid-phase clean-up procedure of these in-vitro samples. In addition, under the conditions described, no endogenous components of the sample interfere at the detection wavelength chosen. After optimization of the separation conditions, some analytical characteristics of the developed CZE method were investigated. A limitof detection of only 0.08 mug/mL was obtained for terbinafine using a standard solution. Finally, the use of on-line CZE/ diode array detection enabled to identify tentatively the presence of unmetabolized parent terbinafineand its metabolites in the mixture of nine components separated.

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Documento generato il 18/01/20 alle ore 07:40:58