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Titolo:
Analysis of loss of heterozygosity on chromosomes 10q, 11, and 16 in medulloblastomas
Autore:
Yin, XL; Pang, JC; Liu, YH; Chong, EY; Cheng, Y; Poon, WS; Ng, HK;
Indirizzi:
Chinese Univ Hong Kong, Prince Wales Hosp, Dept Anat & Cellular Pathol, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China Chinese Univ Hong Kong, Prince Wales Hosp, Dept Surg, Neurosurg Unit, HongKong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China W China Univ Med Sci, Univ Hosp 1, Dept Neurosurg, Chengdu 610041, PeoplesR China W China Univ Med Sci Chengdu Peoples R China 610041 0041, PeoplesR China
Titolo Testata:
JOURNAL OF NEUROSURGERY
fascicolo: 5, volume: 94, anno: 2001,
pagine: 799 - 805
SICI:
0022-3085(200105)94:5<799:AOLOHO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMITIVE NEUROECTODERMAL TUMORS; CENTRAL-NERVOUS-SYSTEM; CHILDHOOD BRAIN-TUMORS; HUMAN LUNG-CANCER; H-CADHERIN CDH13; BREAST-CANCER; MICROSATELLITE ANALYSIS; GENE-MUTATIONS; ALLELIC LOSS; PTEN GENE;
Keywords:
medulloblastoma; loss of heterozygosity; chromosome 16; chromosome 11;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Ng, HK Chinese Univ Hong Kong, Prince Wales Hosp, Dept Anat & Cellular Pathol, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China China
Citazione:
X.L. Yin et al., "Analysis of loss of heterozygosity on chromosomes 10q, 11, and 16 in medulloblastomas", J NEUROSURG, 94(5), 2001, pp. 799-805

Abstract

Object. The loss of genetic material from specific chromosome loci is a common feature in the oncogenesis of tumors and is often indicative of the presence of important tumor suppressor genes at these loci. Recent molecular genetic analyses have demonstrated frequent loss of chromosomes 10q, 11, and 16 in medulloblastomas. The aim of this study was to localize the targeted deletion regions on the three aforementioned chromosomes in medulloblastomas. Methods. Loss of heterozygosity (LOH) was examined on chromosomes 10q, 11,and 16 in a series of 22 primary and two recurrent medulloblastomas by using polymerase chain reaction-based microsatellite analysis. The DNA extracted from the tumors and corresponding normal blood samples were amplified independently in the presence of radioactively labeled microsatellite primers, resolved by denaturing gel electrophoresis and processed for autoradiography. The DNA obtained from control blood samples that displayed allelic heterozygosity at a given microsatellite locus were considered informative. Loss of heterozygosity was inferred when the allelic signal intensity of the tumor sample was reduced by at least 40%, relative to that of the constitutional control. The LOH analysis demonstrated that deletions of chromosomes 10q, lip, and 16q are recur-rent genetic events in the development of medulloblastomas. Three subchromosomal regions of loss have been identified and are localized to the deleted in malignant brain tumors 1 [DMBT1] gene site on chromosomes 10q25, 11p13-11p15.1. and 16q24.1-24.3. Conclusions. These results indicate that DMBT1 is closely associated with the oncogenesis of medulloblastomas and highlight regions of loss on chromosomes 11p and 16q for further fine mapping and cloning of candidate tumor suppressor genes that are important for the genesis of medulloblastoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:38:49