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Titolo:
Ontogeny of P-glycoprotein in mouse intestine, liver, and kidney
Autore:
Mahmood, B; Daood, MJ; Hart, C; Hansen, TWR; Watchko, JF;
Indirizzi:
Univ Pittsburgh, Sch Med, Magee Womens Res Inst, Dept Pediat, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 Pediat, Pittsburgh, PA 15260 USA Univ Oslo, Rikshosp, Dept Pediat, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316 Rikshosp, Dept Pediat, N-0316 Oslo, Norway
Titolo Testata:
JOURNAL OF INVESTIGATIVE MEDICINE
fascicolo: 3, volume: 49, anno: 2001,
pagine: 250 - 257
SICI:
1081-5589(200105)49:3<250:OOPIMI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIDRUG-RESISTANCE GENE; BLOOD-BRAIN-BARRIER; MONOCLONAL-ANTIBODIES; GROWTH-FACTOR; EXPRESSION; MICE; DRUGS; ABSORPTION; TISSUES; DEXAMETHASONE;
Keywords:
development; pharmacology; drug transporters;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Watchko, JF Magee Womens Hosp, Dept Pediat, 300 Halket St, Pittsburgh, PA 15213 USA Magee Womens Hosp 300 Halket St Pittsburgh PA USA 15213 13 USA
Citazione:
B. Mahmood et al., "Ontogeny of P-glycoprotein in mouse intestine, liver, and kidney", J INVES MED, 49(3), 2001, pp. 250-257

Abstract

Background: P-glycoprotein (Pgp) is an ATP-dependent, integral plasma-membrane efflux pump that is constitutively expressed on (i) adult apical brush-herder epithelial cells of the intestine, iii) the bile canalicular face of hepatocytes, and (iii) the brush border epithelium of renal proximal tubules, This Pgp tissue distribution and localization affects the absorption, distribution, metabolism, and excretion of Pgp substrates, Little is known regarding the ontogeny of Pgp expression in these tissues. Methods: Postnatal expression of Pgp on brush border membranes of small intestine, Liver, and kidney as a function of maturity from birth through adulthood was determined using Western immunoblotting and immunohistochemical techniques. Tissue was isolated from FVB mice at four different ages: day of life 0 (D0), day of life 7 (D7, day of life 21 (D21), and adult (Ad). Therelative expression of Pgp protein on Western immunoblots was assessed by scanning densitometry and indexed as a percentage (mean+/-SEM) of the adultlevels,Results: On Western immunoblots, Pgp expression was limited at birth (19+/-6% of Ad) and increased significantly with maturation in intestine (ANOVA,P<0.005). In contrast, hepatic (113+/-12% of Ad) and renal (96+/-15% of Ad) Pgp expression were at adult levels at birth. The tissue-specific developmental pattern of Pgp expression was confirmed by immunohistochemistry,Conclusions: We conclude that Pgp is expressed in a tissue-specific and developmentally regulated fashion and speculate that developmental modulationof intestine-Pgp expression may affect the oral bioavailability of Pgp substrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:24:51