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Titolo:
DIFFERENTIAL INDUCTION OF APOPTOSIS BY VIRULENT MYCOBACTERIUM-TUBERCULOSIS IN RESISTANT AND SUSCEPTIBLE MURINE MACROPHAGES - ROLE OF NITRIC-OXIDE AND MYCOBACTERIAL PRODUCTS
Autore:
ROJAS M; BARRERA LF; PUZO G; GARCIA LF;
Indirizzi:
UNIV ANTIQUIA,FAC MED,LAB CENT INVEST,CTR INVEST MED MEDELLIN COLOMBIA UNIV ANTIQUIA,FAC MED,LAB CENT INVEST,CTR INVEST MED MEDELLIN COLOMBIA CNRS,INST PHARMACOL & BIOL STRUCT TOULOUSE FRANCE
Titolo Testata:
The Journal of immunology
fascicolo: 3, volume: 159, anno: 1997,
pagine: 1352 - 1361
SICI:
0022-1767(1997)159:3<1352:DIOABV>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; PROGRAMMED CELL-DEATH; INTERFERON-GAMMA; INTRACELLULAR PARASITES; NATURAL-RESISTANCE; GENETIC-REGULATION; HUMAN-DISEASE; FACTOR-ALPHA; IFN-GAMMA; BOVIS BCG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
M. Rojas et al., "DIFFERENTIAL INDUCTION OF APOPTOSIS BY VIRULENT MYCOBACTERIUM-TUBERCULOSIS IN RESISTANT AND SUSCEPTIBLE MURINE MACROPHAGES - ROLE OF NITRIC-OXIDE AND MYCOBACTERIAL PRODUCTS", The Journal of immunology, 159(3), 1997, pp. 1352-1361

Abstract

Resistance and susceptibility of macrophages to mycobacteria are under the control of the Bcg/Nramp1 gene, which also controls the NO- production in response to macrophage activators, There is recent evidence indicating that mycobarteria induces apoptosis in infected macrophages. Using murine macrophage lines, congenic at the Bcg/Nramp1 gene, thisreport shows that B10R are more prone than B10S macrophages to undergo apoptosis after exposure to live virulent Mycobacterium tuberculosisH37Rv (Mtb) or PPD, as determined by cell viability, DNA fragmentation, hypoploidy, and the terminal deoxynucleotide transferase dUTP-biotin nick-end labeling assay, induction of apoptosis correlated with NO- production. Aminoguanidine and anti-TNF-a: inhibited NO- production and apoptosis, B10R and B10S macrophages were equally affected by sodiumnitroprusside, a donor of NO-, but its effect, mainly in B10R cells, was enhanced by the presence of Mtb, Nonvirulent mycobacteria induced lower levels of NO- and did not cause cell death, Killed Mtb, mannose-capped lipoarabinomannan (ManLAM), and LPS rescued macrophages from apoptosis albeit induce NO-. These findings suggest the existence of opposite pathways: metabolically active mycobacteria promotes apoptosis whereas,their structural components inhibit it, Apoptosis may be a critical mechanism by which Nramp1 gene controls the macrophage infection with virulent mycobacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:40:13