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Titolo:
Differential expression of CD44 isoforms during liver regeneration in rats
Autore:
Della Fazia, MA; Pettirossi, V; Ayroldi, E; Riccardi, C; Magni, MV; Servillo, G;
Indirizzi:
Univ Perugia, Dipartimento Sci Biochim & Biotecnol Mol, Sez Fisiopatol, Monteluce Policlin, I-06100 Perugia, Italy Univ Perugia Perugia Italy I-06100 luce Policlin, I-06100 Perugia, Italy Univ Perugia, Fac Med & Chirurg, Dipartimento Med Clin & Sperimentale, SezFarmacol, I-06100 Perugia, Italy Univ Perugia Perugia Italy I-06100 , SezFarmacol, I-06100 Perugia, Italy
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 4, volume: 34, anno: 2001,
pagine: 555 - 561
SICI:
0168-8278(200104)34:4<555:DEOCID>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPLICE VARIANTS; GROWTH-FACTOR; HEPATOCELLULAR-CARCINOMA; CELLULAR PROLIFERATION; HEPATIC REGENERATION; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; PARTIAL-HEPATECTOMY; PROTEIN EXPRESSION; DISTANT METASTASIS;
Keywords:
alternative splicing; cell proliferation; liver; hepatoma cells; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Servillo, G Univ Perugia, Dipartimento Sci Biochim & Biotecnol Mol, Sez Fisiopatol, Monteluce Policlin, Succ 3, I-06100 Perugia, Italy Univ Perugia Succ 3 Perugia Italy I-06100 6100 Perugia, Italy
Citazione:
M.A. Della Fazia et al., "Differential expression of CD44 isoforms during liver regeneration in rats", J HEPATOL, 34(4), 2001, pp. 555-561

Abstract

Background: CD44 is a transmembrane glycoprotein known to bind hyaluronic acid (HA). This molecule is a multi-functional cell surface glycoprotein involved in lymphocyte homing and activation, tumor growth and metastasis. Wehave investigated the qualitative modification of CD44 in the regeneratingliver as a model for studying cellular proliferation in vivo. Molecules involved in cell adhesion and the extracellular matrix (ECM), which influencedifferentiation, growth, cell-cell interactions and cellular polarity, play an important role in the liver regeneration. We studied the modulation ofCD44 gene expression and its post-transcriptional modifications, analyzingthe expression of different isoforms containing exon v6 in the regenerating liver, in sham operated liver and in the hepatoma cells H-35. Methods: The expression of CD44 and CD44v6 were analyzed in RNA extracted from regenerating liver at different times after partial hepatectomy (PH), and H-35 hepatoma cells by Northern blot, RT-PCR and Southern blot, and in protein extracts from regenerating liver by Western blot. H-35 hepatoma cells were assayed with the antibody cross-linked technique with CD44 antibodies. Results: The standard CD44 form is expressed in regenerating liver and itslevels were not modified following PH. However, our analysis revealed CD44isoforms containing v6 in the first hours after PH as well as in the H-35 hepatoma cell line. H-35 cells treated with cross-linked anti-CD44 antibodies or HA show an increased rate of incorporation of [H-3]thymidine (30 and 25%, respectively) with respect to the control. Conclusion: These findings suggest that CD44 may play a role in the proliferation of residual hepatocytes following PH. (C) 2001 European Associationfor the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 11:37:03