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Titolo:
CD133(+) cell selection is an alternative to CD34(+) cell selection for exvivo expansion of hematopoietic stem cells
Autore:
Kobari, L; Giarratana, MC; Pflumio, F; Izac, B; Coulombel, L; Douay, L;
Indirizzi:
Hop Armand Trousseau, Serv Hematol Biol, F-75012 Paris, France Hop Armand Trousseau Paris France F-75012 ol Biol, F-75012 Paris, France Hop St Antoine, INSERM, U417, F-75571 Paris, France Hop St Antoine ParisFrance F-75571 INSERM, U417, F-75571 Paris, France ICGM, INSERM, U474, Paris, France ICGM Paris FranceICGM, INSERM, U474, Paris, France
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 2, volume: 10, anno: 2001,
pagine: 273 - 281
SICI:
1525-8165(200104)10:2<273:CCSIAA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURE-INITIATING CELLS; IMMUNE-DEFICIENT MICE; BONE-MARROW CELLS; HUMAN CORD BLOOD; PROGENITOR CELLS; IN-VITRO; STROMA; DIFFERENTIATION; IDENTIFICATION; REPOPULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Douay, L Hop Armand Trousseau, Serv Hematol Biol, 26 Ave Dr Arnold Netter,F-75012 Paris, France Hop Armand Trousseau 26 Ave Dr Arnold Netter Paris France F-75012
Citazione:
L. Kobari et al., "CD133(+) cell selection is an alternative to CD34(+) cell selection for exvivo expansion of hematopoietic stem cells", J HEMATH ST, 10(2), 2001, pp. 273-281

Abstract

CD133 is a new stem cell antigen that may provide an alternative to CD34 for the selection and expansion of hematopoietic cells for transplantation. This study compared the expansion capacities of CD133(+) and CD34(+) cells isolated from the same cord blood (CB) samples. After 14 days culture in stroma-free, serum-free medium in the presence of stem cell factor (SCF), Flt3-1, megakaryocyte growth and development factor (MGDF), and granulocyte colony-stimulating factor (G-CSF), the CD133(+) and CD34(+) fractions displayed comparable expansion of the myeloid compartment (CFC, LTC-IC, and E-LTC-IC). The expansion of CD133(+) CB cells was up to 1262-fold for total cells, 99-fold for CD34(+) cells, 109-fold for CD34(+) CD133(+) cells, 133-fold for CFU-GM, 14.5-fold for LTC-IC, and 7.5-fold for E-LTC-IC. Moreover, the expanded population was able to generate lymphoid B (CD19(+)), NK (CD56(+)), and T (CD4(+)CD8(+)) cells in liquid or fetal thymic organ cultures, while expression of the homing antigen CXCR4 was similar on expanded and nonexpanded CD133(+) or CD34(+) cells. Thus, the CD133(+) subset could be expanded in the same manner as the CD34(+) subset and conserved its multilineage capacity, which would support the relevance of CD133 for clinical hematopoietic selection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 18:05:17