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Titolo:
Phenotypic differentiation of TGF-beta 1-responsive pluripotent premesenchymal prehematopoietic progenitor (P4 stem) cells from murine bone marrow
Autore:
Hall, FL; Han, B; Kundu, RK; Yee, A; Nimni, ME; Gordon, EM;
Indirizzi:
Univ So Calif, Keck Sch Med, Gene Therapy Labs, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 py Labs, Los Angeles, CA 90089 USA Univ So Calif, Keck Sch Med, Dept Surg, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 pt Surg, Los Angeles, CA 90089 USA Univ So Calif, Keck Sch Med, Dept Pediat, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Pediat, Los Angeles, CA 90089 USA Univ So Calif, Keck Sch Med, Dept Biochem, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Biochem, Los Angeles, CA 90089 USA Univ So Calif, Keck Sch Med, Dept Mol Pharmacol Toxicol, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Toxicol, Los Angeles, CA 90089 USA
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 2, volume: 10, anno: 2001,
pagine: 261 - 271
SICI:
1525-8165(200104)10:2<261:PDOT1P>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUSION PROTEIN; MESENCHYMAL CELLS; IN-VIVO; COLLAGEN; EXPRESSION; REGENERATION; REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Gordon, EM Univ So Calif, Keck Sch Med, Gene Therapy Labs, Los Angeles, CA90089 USA Univ So Calif Los Angeles CA USA 90089 s Angeles, CA 90089 USA
Citazione:
F.L. Hall et al., "Phenotypic differentiation of TGF-beta 1-responsive pluripotent premesenchymal prehematopoietic progenitor (P4 stem) cells from murine bone marrow", J HEMATH ST, 10(2), 2001, pp. 261-271

Abstract

On the horizon of modern molecular medicine is the requisite technology tocapture multipotent human stem cells that are capable of self-renewal and to direct these stem cells along defined lineages for therapeutic purposes. In this article, we describe the hematopoietic and mesenchymal differentiation potential of a unique population of transforming growth factor-beta1 (TGF-beta1)-responsive stem cells derived from murine bone marrow. Stringentselection of the stem cells was accomplished under low serum conditions byvirtue of an inherent survival response to a TGF-beta1-vWF fusion protein that was bound to collagen matrices. The TGF-beta1-responsive stem cells initially exhibited a nonadherent and uniformly blastoid morphology, underwent expansion into colonies upon serum reconstitution, and were capable of overt cytodifferentiation along fibrogenic, osteogenic, chondrogenic, or adipogenic lineages upon growth factor stimulation. Remarkably, these stem cells also underwent rapid expansion in the presence of either hematopoietic stem cell factor (SCF) or interleukin-3 (IL-3), and differentiated into myeloid and lymphoid phenotypes upon exposure to the latter. Taken together, these results support the hypothesis that pluripotent premesenchymal prehematopoietic progenitor cells, designated P4 stem cells, are present postnatallyin murine bone marrow and, thus, may be summarily isolated for various cell-based experimental therapies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:31:05