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Titolo:
Flow-cytometric analysis of lymphocyte subsets and mCD14 expression in patients with various periodontitis categories
Autore:
Buduneli, N; Bicakci, N; Keskinoglu, A;
Indirizzi:
Ege Univ, Fac Dent, Dept Periodontol, TR-35100 Izmir, Turkey Ege Univ Izmir Turkey TR-35100 Dept Periodontol, TR-35100 Izmir, Turkey Ege Univ, Fac Med, Dept Paediat, Izmir, Turkey Ege Univ Izmir TurkeyEge Univ, Fac Med, Dept Paediat, Izmir, Turkey
Titolo Testata:
JOURNAL OF CLINICAL PERIODONTOLOGY
fascicolo: 5, volume: 28, anno: 2001,
pagine: 419 - 424
SICI:
0303-6979(200105)28:5<419:FAOLSA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE; EARLY-ONSET PERIODONTITIS; SOLUBLE CD14; ADULT PERIODONTITIS; CYTOKINE PRODUCTION; MONONUCLEAR-CELLS; ESCHERICHIA-COLI; BINDING-PROTEIN; NEUTROPHILS; FIBROBLASTS;
Keywords:
mCD14; lymphocyte subsets; flow cytometry; lipopolysaccharides; periodontitis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Buduneli, N Ege Univ, Fac Dent, Dept Periodontol, TR-35100 Izmir, Turkey Ege Univ Izmir Turkey TR-35100 ontol, TR-35100 Izmir, Turkey
Citazione:
N. Buduneli et al., "Flow-cytometric analysis of lymphocyte subsets and mCD14 expression in patients with various periodontitis categories", J CLIN PER, 28(5), 2001, pp. 419-424

Abstract

Background: Membrane-bound CD14 (mCD14) is expressed mainly on circulatingmonocytes and tissue macrophages. It is one of the receptors, which act atthe recognition of lipopolysaccharides by host cells. Periodontopathic bacteria result in activation of cellular and humoral immune responses. Aim: The aim of the present study was to analyze the peripheral blood mCD14 concentrations as well as cell surface markers of lymphocyte subsets in periodontitis patients of various categories. Materials and Methods: Peripheral blood samples were obtained from 22 early onset periodontitis (EOP), 10 adult periodontitis (AP) patients and 13 systemically and periodontally healthy control subjects, Three-color how cytometry and st panel of relevant monoclonal antibodies were used to determinethe percent expression of various cell surface markers on peripheral bloodmononuclear cells (PBMCs). The results were tested statistically by one-way variance analysis and Newman Keuls test. Results: No significant difference was observed between the study groups with regard to the relative counts of B-cells, T-cells, T-helper, T-cytotoxic/suppressor, activated T-cells and natural killer cells. EOP patients expressed significantly lower level of interleukin-2 receptor (IL-2R) when compared with PLP patients (6.08% and 19.3% respectively) (p<0.05). The level of mCD14 in EOP patients (7.18%) was lower than that of AP patients (9.3%) and the control subjects (9.2%), but the differences were not statistically significant. Conclusions: The low level of IL-2R in the EOP group may be interpreted asan insufficient responsiveness to the periodontopathogens, which may be ultimately related with the more severe tissue destruction. Though not significant, the reduced expression of mCD14 in EOP group may also be related with the immune system deficiencies in these patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:03:02