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Titolo:
Secondary typing of Mycobacterium tuberculosis isolates with matching IS6110 fingerprints from different geographic regions of the United States
Autore:
Yang, ZH; Bates, JH; Eisenach, KD; Cave, MD;
Indirizzi:
Cent Arkansas Vet Healthcare Syst, Med Res Serv, Reg TB Genotyping Lab, Little Rock, AR 72205 USA Cent Arkansas Vet Healthcare Syst Little Rock AR USA 72205 , AR 72205 USA Univ Arkansas Med Sci, Dept Internal Med, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Univ Arkansas Med Sci, Dept Immunol Microbiol, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Univ Arkansas Med Sci, Dept Pathol, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Univ Arkansas Med Sci, Dept Anat, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Arkansas Dept Hlth, Little Rock, AR 72205 USA Arkansas Dept Hlth Little Rock AR USA 72205 th, Little Rock, AR 72205 USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 1691 - 1695
SICI:
0095-1137(200105)39:5<1691:STOMTI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; FALSE-POSITIVE CULTURES; NOSOCOMIAL TRANSMISSION; STRAIN DIFFERENTIATION; MOLECULAR EPIDEMIOLOGY; EXOGENOUS REINFECTION; CROSS-CONTAMINATION; DNA POLYMORPHISM; COMPLEX STRAINS; SAN-FRANCISCO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Cave, MD Cent Arkansas Vet Healthcare Syst, Med Res Serv, Reg TB Genotyping Lab, LR-151,4300 W 7th St, Little Rock, AR 72205 USA Cent Arkansas Vet Healthcare Syst LR-151,4300 W 7th St Little Rock AR USA 72205
Citazione:
Z.H. Yang et al., "Secondary typing of Mycobacterium tuberculosis isolates with matching IS6110 fingerprints from different geographic regions of the United States", J CLIN MICR, 39(5), 2001, pp. 1691-1695

Abstract

Fifty-nine isolates of Myeobacterium tuberculosis obtained from different states in the United States and representing 25 interstate clusters were investigated. These clusters were identified by computer-assisted analysis ofDNA fingerprints submitted during 1996 and 1997 by different laboratories participating in the CDC National Genotyping and Surveillance Network. Isolates were fingerprinted with the IS6110 right-hand probe (IS6110-3'), the IS6110 left-hand probe (IS6110-5'), and the probe pTBN12, containing the polymorphic GC-rich sequence (PGRS), Spoligotyping based on the polymorphism in the 36-bp direct-repeat locus was also performed. As a control, 43 M. tuberculosis isolates in 17 clusters obtained from patients in Arkansas duringthe study period were analyzed. Of the 25 interstate clusters, 19 were confirmed as correctly clustered when all the isolates were analyzed on the same gel using the IS6110-3' probe. Of the 19 true IS6110-3' clusters, 10 (53%) were subdivided by one or more secondary typing methods. Clustering of the control group was virtually identical by all methods. Of the three different secondary typing methods, spoligotyping was the least discriminating. IS6110-5' fingerprinting aas as discriminating as PGRS fingerprinting. The data indicate that the IS6110-5' probe not only is a useful secondary typing method but also probably would prove to be a more useful primary typing method for a genotyping network which involves isolates from different geographic regions.

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Documento generato il 29/03/20 alle ore 01:59:23