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Titolo:
Binding and uptake of differently oxidized low density lipoprotein in mouse peritoneal macrophages and THP-1 macrophages: Involvement of negative charges as well as oxidation-specific epitopes
Autore:
Wang, XS; Greilberger, J; Ledinski, G; Kager, G; Jurgens, G;
Indirizzi:
Karl Franzens Univ Graz, Inst Med Chem, A-8010 Graz, Austria Karl FranzensUniv Graz Graz Austria A-8010 d Chem, A-8010 Graz, Austria Karl Franzens Univ Graz, Inst Med Chem & Mol Biol, A-8010 Graz, Austria Karl Franzens Univ Graz Graz Austria A-8010 l Biol, A-8010 Graz, Austria Karl Franzens Univ Graz, Pregl Lab, A-8010 Graz, Austria Karl Franzens Univ Graz Graz Austria A-8010 gl Lab, A-8010 Graz, Austria
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 3, volume: 81, anno: 2001,
pagine: 557 - 569
SICI:
0730-2312(2001)81:3<557:BAUODO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED RECOGNITION; HUMAN MONOCYTE MACROPHAGES; SCAVENGER RECEPTOR; APOPTOTIC THYMOCYTES; SERUM-LIPOPROTEINS; MURINE MACROPHAGES; PROTEIN OXIDATION; MEMBRANE-PROTEIN; LDL RECEPTOR; FATTY-ACIDS;
Keywords:
low-density lipoprotein; macrophage; oxidative modification; lipoprotein metabolism; monoclonal antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Jurgens, G Karl Franzens Univ Graz, Inst Med Chem, Harrachgasse 21, A-8010Graz, Austria Karl Franzens Univ Graz Harrachgasse 21 Graz Austria A-8010ia
Citazione:
X.S. Wang et al., "Binding and uptake of differently oxidized low density lipoprotein in mouse peritoneal macrophages and THP-1 macrophages: Involvement of negative charges as well as oxidation-specific epitopes", J CELL BIOC, 81(3), 2001, pp. 557-569

Abstract

Oxidatively modified low-density lipoprotein (LDL) has been found in vivo,and oxidized LDL (oxLDL) could bind to scavenger receptors, leading to foam cell formation. Macrophages bear a number of different scavenger receptors for oxLDL, and macrophages of different origins may have a different scavenger receptor repertoire. In addition, LDL oxidized to different degrees may differ in the ability to bind macrophage scavenger receptors. In this study, we characterized the patterns of the binding and uptake of differentlyoxidized LDL in mouse peritoneal macrophages (MPM) and human THP-1 macrophages, and the influence of negative charge and oxidation-specific epitopes in oxLDL on these processes. Thresholds of increased binding and uptake in MPM were found when LDL was oxidized to the degrees with a relative electrophoretic mobility (REM) of 2.6 (minor threshold) and 3.0 (major threshold),corresponding to 49 and 57%, respectively, of the loss of free amino groups in these oxLDL. There was no threshold for the binding of oxLDL to THP-1 macrophages, while for uptake, a major threshold with REM of 3.0 (57% free amino groups lost) was found. The presence of the F(ab')(2) fragments of the monoclonal antibody OB/04, which was raised against copper-oxidized LDL, led to the reduction of the binding and uptake, respectively, of Eu3+-oxLDL(REM:3.6) in MPM by 31 and 29%, and by 19 and 22% in THP-1 macrophages. Itis concluded that LDL oxidized to different degrees binds differently to macrophages, and the patterns of binding and uptake are different for MPM and human THP-1 macrophages. Both, the negative charge and the oxidation-specific epitopes of oxLDL are involved in these processes. J. Cell. Biochem. 81:557-569, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/02/20 alle ore 23:39:21