Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Indole-3-propionic acid, a melatonin-related molecule, protects hepatic microsomal membranes from iron-induced oxidative damage: Relevance to cancer reduction
Autore:
Karbownik, M; Reiter, RJ; Garcia, JJ; Cabrera, J; Burkhardt, S; Osuna, C; Lewinski, A;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 truct Biol, San Antonio, TX 78229 USA Med Univ Lodz, Inst Endocrinol, Dept Thyroidol, Lodz, Poland Med Univ Lodz Lodz Poland Inst Endocrinol, Dept Thyroidol, Lodz, Poland
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 3, volume: 81, anno: 2001,
pagine: 507 - 513
SICI:
0730-2312(2001)81:3<507:IAAMMP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPID-PEROXIDATION PRODUCTS; DELTA-AMINOLEVULINIC-ACID; IN-VIVO; FLUIDITY; DNA; STRESS; INVOLVEMENT; METABOLITES; MECHANISMS; SYSTEM;
Keywords:
indole-3-propionic acid; iron; cancer; membrane fluidity; lipid peroxidation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Reiter, RJ Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, Mail Code 7762,7703Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas Mail Code 7762,7703 Floyd Curl Dr San Antonio TX USA 78229
Citazione:
M. Karbownik et al., "Indole-3-propionic acid, a melatonin-related molecule, protects hepatic microsomal membranes from iron-induced oxidative damage: Relevance to cancer reduction", J CELL BIOC, 81(3), 2001, pp. 507-513

Abstract

Excessive free iron and the associated oxidative damage are commonly related to carcinogenesis. Among the antioxidants known to protect against iron-induced oxidative abuse and carcinogenesis, melatonin and other indole compounds recently have received considerable attention. Indole-3-propionic acid (IPA), a deamination product of tryptophan, with a structure similar to that of melatonin, is present in biological fluids and is an effective free radical scavenger. The aim of the study was to examine the effect of IPA onexperimentally induced oxidative changes in rat hepatic microsomal membranes. Microsomes were preincubated in presence of IPA (10, 3, 2, 1, 0.3, 0.1,0.01 or 0.001 mM) and, then, incubated with FeCl3 (0.2 mM), ADP (1.7 mM) and NADPH (0.2 mM) to induce oxidative damage. Alterations in membrane fluidity (the inverse of membrane rigidity) were estimated by fluorescence spectroscopy and lipid peroxidation by measuring concentrations of malondialdehyde+4-hydroxyalkenals (MDA+4-HDA). IPA, when used in concentrations of 10, 3or 2 mM, increased membrane fluidity, although at these concentrations ii did not influence lipid peroxidation significantly. The decrease in membrane fluidity due to Fe3+ was completely prevented by preincubation in the presence of IPA at concentrations of 10, 3, 2 or 1 mM. The enhanced lipid peroxidation due to Fe3+ was prevented by IPA only at the highest concentration(10 mM). It is concluded that Fe3+-induced rigidity and, to a lesser extent, lipid peroxidation in microsomal membranes may be reduced by IPA. However, IPA in high concentrations increase membrane fluidity. Besides melatonin, IPA may be used as a pharmacological agent to protect against iron-induced oxidative damage to membranes and, potentially, against carcinogenesis, J. Cell. Biochem. 81:507-513, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:52:48