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Titolo:
Effect of the neuropeptide substance P on the rat bone marrow-derived osteogenic cells in vitro
Autore:
Adamus, MA; Dabrowski, ZJ;
Indirizzi:
Jagiellonian Univ, Inst Zool, Dept Anim Physiol, PL-30060 Krakow, Poland Jagiellonian Univ Krakow Poland PL-30060 hysiol, PL-30060 Krakow, Poland
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 3, volume: 81, anno: 2001,
pagine: 499 - 506
SICI:
0730-2312(2001)81:3<499:EOTNSP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED PEPTIDE; STROMAL CELLS; OSTEOBLASTIC PHENOTYPE; SURGICAL SYMPATHECTOMY; MESSENGER-RNAS; ADULT RATS; RESORPTION; DEXAMETHASONE; INDUCTION; RECEPTOR;
Keywords:
substance P; osteogenic cells; mineralization; alkaline phosphatase; dexamethasone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Adamus, MA Jagiellonian Univ, Inst Zool, Dept Anim Physiol, Ingardena 6, PL-30060 Krakow, Poland Jagiellonian Univ Ingardena 6 Krakow Poland PL-30060 w, Poland
Citazione:
M.A. Adamus e Z.J. Dabrowski, "Effect of the neuropeptide substance P on the rat bone marrow-derived osteogenic cells in vitro", J CELL BIOC, 81(3), 2001, pp. 499-506

Abstract

Substance P containing, thin, sensory nerve fibres have been demonstrated in bone and bone marrow. However the role of substance P in bone tissue is not fully understood. We investigated the effects of substance P on the growth and development of rat bone marrow-derived osteogenic cells in vitro. To examine this, the marrow-derived osteogenic cells were treated from 3rd to 6th day of subculture with substance P at concentrations 10(-10), 10(-9) and 10(-8)M. [H-3]-thymidine, L-2,3-[H-3]-proline incorporation, protein accumulation, alkaline phosphatase activity, and calcium deposition were measured in cultures. Substance P slightly stimulated [3H]-thymidine incorporation at 10(-10) M. Protein accumulation and L-2,3-[H-3]-proline incorporation were enhanced in a dose dependent manner. Simultaneous application of spantide, a substance P receptor antagonist, could not block substance P-induced L-2,3-[H-3]-proline incorporation probably because of statistically significant effect of spantide itself. Calcium deposits were significantly lower (about 30%) in cultures treated with SP. This effect was probably due in part by the fall in alkaline phosphatase activity which in substance P treated cultures was decreased about 17%. Our results indicate that substance Pcould be one of the factors modulating bone metabolism. J. Cell. Biochem. 81:499-506, 2001. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 23/01/21 alle ore 10:19:10