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Titolo:
Cyclic strain induces reactive oxygen species production via an endothelial NAD(P)H oxidase
Autore:
Matsushita, H; Lee, KH; Tsao, PS;
Indirizzi:
Stanford Univ, Sch Med, Div Cardiovasc Med, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ardiovasc Med, Stanford, CA 94305 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
, , anno: 2001, supplemento:, 36
pagine: 99 - 106
SICI:
0730-2312(2001):<99:CSIROS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOTACTIC PROTEIN-1; NECROSIS-FACTOR-ALPHA; GENE-EXPRESSION; SMOOTH-MUSCLE; SIGNAL-TRANSDUCTION; CELLS; ACTIVATION; P22(PHOX); GROWTH; ATHEROSCLEROSIS;
Keywords:
superoxide anion; protein kinase C; endothelium; hemodynamic; cyclic stretch;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Tsao, PS Stanford Univ, Sch Med, Div Cardiovasc Med, 300 Pasteur Dr, Stanford, CA 94305 USA Stanford Univ 300 Pasteur Dr Stanford CA USA 94305 , CA 94305 USA
Citazione:
H. Matsushita et al., "Cyclic strain induces reactive oxygen species production via an endothelial NAD(P)H oxidase", J CELL BIOC, 2001, pp. 99-106

Abstract

Vascular endothelial cells are constantly subjected to pressure-induced cyclic strain. Reactive oxygen species (ROS) have been implicated in atherosclerosis and Vascular remodeling. Recent evidence indicates that a vascular NAD(P)H oxidase may be an important source of ROS in both physiologic and pathophysiologic situations. The aim of this study was to investigate cyclicstrain-induced NAD(P)H oxidase activity in endothelial cells. ROS production was examined by electron paramagnetic resonance and lucigenin chemiluminescence. Cyclic strain-induced NAD(P)H oxidase activity was quantified by activity assay while the expression of p22phox was monitored by Northern blotting. Endothelial cells produce basal amounts of ROS that were enhanced bycyclic strain. Moreover subsequent stimulation with TNF-alpha resulted in significantly greater ROS production in cells previously exposed to cyclic strain as compared to static conditions. Cyclic strain resulted in a significant increase in message for the p22phox subunit as well as activity of the NAD(P)H oxidase. The induced oxidative stress was accompanied by increased mobilization of the transcription factor NF kappaB, an effect that was blocked by a pharmacological inhibitor of NAD(P)H. These results demonstrate a pivotal role for NAD(P)H oxidase in cyclic strain-induced endothelial ROSproduction and may provide insight into the modulation of vascular diseaseby biomechanical forces. (C) 2001 Wiley-Liss. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 10:10:42