Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MEKK2 is required for T-cell receptor signals in JNK activation and interleukin-2 gene expression
Autore:
Su, B; Cheng, JK; Yang, JH; Guo, ZJ;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA UnivTexas Houston TX USA 77030 Ctr, Dept Immunol, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 18, volume: 276, anno: 2001,
pagine: 14784 - 14790
SICI:
0021-9258(20010504)276:18<14784:MIRFTR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-TERMINAL KINASE; PROTEIN-KINASE; C-JUN; MOLECULAR-CLONING; PATHWAYS; TRANSDUCTION; MKK7; P38; IDENTIFICATION; SAPK/JNK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Su, B Univ Texas, MD Anderson Canc Ctr, Dept Immunol, Box 178,1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas Box 178,1515 Holcombe Blvd HoustonTX USA 77030 77030 USA
Citazione:
B. Su et al., "MEKK2 is required for T-cell receptor signals in JNK activation and interleukin-2 gene expression", J BIOL CHEM, 276(18), 2001, pp. 14784-14790

Abstract

The c-Jun N-terminal kinases (JNKs) are members of the mitogen-activated protein kinase (MAPK) gene family and are essential for cell proliferation, differentiation, and apoptosis. Previously we found that activation of JNK in T-cells required costimulation of both T-cell receptor and auxiliary receptors such as CD28, In this study, we cloned a full-length human MEK kinase (MEKK) 2 cDNA from Jurkat T-cells and demonstrated that it was a major upstream MAPK kinase kinase for the JNK cascade in T-cells. The human MEKK2 cDNA encoded a polypeptide of 619 amino acids and was the human counterpart of the reported murine MEKK2, It was 94% homologous with human and murine MEKK3 at the catalytic domains and 60% homologous at the N-terminal noncatalytic region. Northern blot analysis showed that MEKK2 was ubiquitously expressed, with the highest level in peripheral blood leukocytes, In T cells, MEKK2 was found to be a strong activator of JNK but not of extracellular signal-regulated kinase MAPKs and to activate JNK-dependent AP-1 reporter geneexpression. MEKK2 also synergized with anti-CD3 antibody to activate JNK in T cells, and stimulation of T cells led to induction of MEKK2 tyrosine phosphorylation, Significantly, the JNK activation induced by anti-CDS and anti-CD28 antibodies, but not by 12-O-tetradecanoylphorbol-13-acetate and Ca2 ionophore A23187, was inhibited by dominant negative MEKK2 mutants. AP-1 and interleukin-2 reporter gene induction in T-cells was also inhibited by dominant negative MEKK2 mutants. Taken together, our results showed that human MEKK2 is a key signaling molecule for T-cell receptor/CD3-mediated JNK MAPK activation and interleukin-2 gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 05:51:48