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Titolo:
Secretion of surfactant protein C, an integral membrane protein, requires the N-terminal propeptide
Autore:
Conkright, JJ; Bridges, JP; Na, CL; Voorhout, WF; Trapnell, B; Glasser, SW; Weaver, TE;
Indirizzi:
Childrens Hosp, Med Ctr, Div Pulm Biol, Cincinnati, OH 45229 USA ChildrensHosp Cincinnati OH USA 45229 ulm Biol, Cincinnati, OH 45229 USA Univ Utrecht, Dept Funct Morphol, NL-3508 TD Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TD 3508 TD Utrecht, Netherlands
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 18, volume: 276, anno: 2001,
pagine: 14658 - 14664
SICI:
0021-9258(20010504)276:18<14658:SOSPCA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYPEPTIDE SP-C; RAT TYPE-II; B SP-B; STRUCTURAL REQUIREMENTS; MULTIVESICULAR ENDOSOMES; CYTOPLASMIC TAIL; LUNG-FUNCTION; PC12 CELLS; RECEPTOR; CONTAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Weaver, TE Childrens Hosp, Med Ctr, Div Pulm Biol, 3333 Burnet Ave, Cincinnati, OH 45229 USA Childrens Hosp 3333 Burnet Ave Cincinnati OH USA 45229 5229 USA
Citazione:
J.J. Conkright et al., "Secretion of surfactant protein C, an integral membrane protein, requires the N-terminal propeptide", J BIOL CHEM, 276(18), 2001, pp. 14658-14664

Abstract

Proteolytic processing of surfactant protein C (SP-C) proprotein in multivesicular bodies of alveolar type II cells results in a 35-residue mature peptide, consisting of a transmembrane domain and a 10-residue extramembrane domain. SP-C mature peptide is stored in lamellar bodies (a lysosomal-like organelle) and secreted with surfactant phopholipids into the alveolar space. This study was designed to identify the peptide domain of SP-C required for sorting and secretion of this integral membrane peptide. Deletion analyses in transiently transfected PC12 cells and isolated mouse type II cells suggested the extramembrane domain of mature SP-C was cytosolic and sufficient for sorting to the regulated secretory pathway. Intratracheal injectionof adenovirus encoding SP-C mature peptide resulted in secretion into the alveolar space of wild type mice but not SP-C (-/-) mice, SP-C secretion innull mice was restored by the addition of the N-terminal propeptide, The cytosolic domain, consisting of the N-terminal propeptide and extramembrane domain of mature SP-C peptide, supported secretion of the transmembrane domain of platelet-derived growth factor receptor, Collectively, these studiesindicate that the N-terminal propeptide of SP-C is required for intracellular sorting and secretion of SP-C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 23:51:53