Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mutagenic and nonmutagenic bypass of DNA lesions by Drosophila DNA polymerases dpol eta and dpol iota
Autore:
Ishikawa, T; Uematsu, N; Mizukoshi, T; Iwai, S; Iwasaki, H; Masutani, C; Hanaoka, F; Ueda, R; Ohmori, H; Todo, T;
Indirizzi:
Kyoto Univ, Ctr Radiat Biol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 diat Biol, Sakyo Ku, Kyoto 6068501, Japan Osaka Univ, Microbial Dis Res Inst, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 es Inst, Suita, Osaka 5650871, Japan Osaka Univ, Inst Mol & Cellular Biol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ar Biol, Suita, Osaka 5650871, Japan Biomol Engn Res Inst, Osaka 5650874, Japan Biomol Engn Res Inst Osaka Japan 5650874 Res Inst, Osaka 5650874, Japan Mitsubishi Kasei Inst Life Sci, Tokyo 1948511, Japan Mitsubishi Kasei InstLife Sci Tokyo Japan 1948511 Tokyo 1948511, Japan Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 niv, Inst Virus Res, Kyoto 6068507, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 18, volume: 276, anno: 2001,
pagine: 15155 - 15163
SICI:
0021-9258(20010504)276:18<15155:MANBOD>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMINE-THYMINE DIMER; ESCHERICHIA-COLI; CIS-SYN; CYCLOBUTANE DIMER; GENE ENCODES; 6-4 ADDUCT; PHOTOPRODUCT; FIDELITY; PROTEIN; REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Todo, T Kyoto Univ, Ctr Radiat Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan Kyoto Univ Yoshidakonoe Cho Kyoto Japan 6068501 to 6068501, Japan
Citazione:
T. Ishikawa et al., "Mutagenic and nonmutagenic bypass of DNA lesions by Drosophila DNA polymerases dpol eta and dpol iota", J BIOL CHEM, 276(18), 2001, pp. 15155-15163

Abstract

cDNA sequences were identified and isolated that encode Drosophila homologues of human Rad30A and Rad30B called drad30A and drad30B, Here we show that the C-terminal-truncated forms of the drad30A and drad30B gene products, designated dpol eta DeltaC and dpol iota DeltaC, respectively, exhibit DNA polymerase activity, dpol eta DeltaC and dpol iota DeltaC efficiently bypass a cis syn.-cyclobutane thymine-thymine (TT) dimer in a mostly error-free manner. dpol eta DeltaC shows limited ability to bypass a 6-4-photoproduct ((6-4)PP) at thymine-thymine (TT-(6-4)PP) or at thymine-cytosine (TC-(6-4)PP) in an error-prone manner. dpol iota DeltaC scarcely bypasses these lesions. Thus, the fidelity of translesion synthesis depends on the identity of the lesion and on the polymerase. The human XPV gene product, hpol eta, bypasses cis-syn-cyclobutane thymine-thymine dimer efficiently in a mostly error-free manner but does not bypass TT-(6-4)PP, whereas Escherichia coli DNApolymerase V (UmuD'C-2 complex) bypasses both lesions, especially TT-(6-4)PP, in an error prone manner (Tang, M., Pham, P., Shen, X, Taylor, J. S., O'Donnell, M., Woodgate, R., and Goodman, M, F. (2000) Nature 404, 1014-1018); Both dpol eta DeltaC and DNA polymerase V preferentially incorporate GA opposite TT-(6-4)PP. The chemical structure of the lesions and the similarity in the nucleotides incorporated suggest that structural information in the altered bases contribute to nucleotide selection during incorporation opposite these lesions by these polymerases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:57:48