Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Selective expression of erg isoforms In human endothelial cells
Autore:
Hewett, PW; Nishi, K; Daft, EL; Murray, JC;
Indirizzi:
Univ Nottingham, City Hosp, CRC Dept Clin Oncol, Mol Oncol Lab, NottinghamNG5 1PB, England Univ Nottingham Nottingham England NG5 1PB b, NottinghamNG5 1PB, England Okayama Dent Sch, Dept Prevent Dent, Shikato, Okayama 700, Japan Okayama Dent Sch Shikato Okayama Japan 700 t, Shikato, Okayama 700, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 4, volume: 33, anno: 2001,
pagine: 347 - 355
SICI:
1357-2725(200104)33:4<347:SEOEII>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETS TRANSCRIPTION FACTORS; GENE-EXPRESSION; CORE PROMOTER; FAMILY MEMBER; BINDING; FLI-1; PROTEINS; ONCOGENE; DIFFERENTIATION; STABILIZATION;
Keywords:
erg; fli-1; ets transcription factors; endothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Hewett, PW Univ Nottingham, City Hosp, CRC Dept Clin Oncol, Mol Oncol Lab,Hucknall Rd, Nottingham NG5 1PB, England Univ Nottingham Hucknall Rd Nottingham England NG5 1PB England
Citazione:
P.W. Hewett et al., "Selective expression of erg isoforms In human endothelial cells", INT J BIO C, 33(4), 2001, pp. 347-355

Abstract

Erg and Fli-1 are closely related members of the ets family of transcription factors. There are at least live human Erg isoforms (Erg-1, Erg-2, Erg-3/p55(Erg), p49(Erg) and p38(Erg)) produced through differential mRNA splicing and alternative use of translational start codons. However, relatively little is known about the expression or function of these isoforms in vitro or their distribution in vivo. We used RT-PCR to screen a panel of primary and established human cell lines for erg and fli-1 consensus sequences. Whilst fli-1 was expressed in several human cell types, erg was detected mainly in endothelial cells. To identify which erg isoforms are expressed in endothelial cells we used RT-PCR, Northern blotting and 5'-RACE. Erg-3/p55(Erg) and p38(Erg)/p38(Erg)-like transcripts were detected in both microvascular and large vessel endothelial cells affinity-purified from different vascular beds. Moreover, these erg isoforms were present in both freshly isolated, and confluent endothelial cells following several passages in culture, indicating that endothelial erg expression in vitro may be broadly representative of that in vivo. The selective expression of the Erg-3/p55(Erg) and p38(Erg)/p38(Erg)-like isoforms in endothelial cells indicates their involvement in the regulation of endothelial-restricted genes. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:03:55