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Titolo:
A powerful and rapid approach to human genome scanning using small quantities of genomic DNA
Autore:
Beekman, M; Lakenburg, N; Cherny, SS; De Knijff, P; Kluft, CC; Van Ommen, GJB; Vogler, GP; Frants, RR; Boomsma, DI; Slagboom, PE;
Indirizzi:
TNO Prevent & Hlth, Gaubius Lab, NL-2301 CE Leiden, Netherlands TNO Prevent & Hlth Leiden Netherlands NL-2301 CE CE Leiden, Netherlands Leiden Univ, Med Ctr, Dept Human Genet, Leiden, Netherlands Leiden Univ Leiden Netherlands r, Dept Human Genet, Leiden, Netherlands Inst Psychiat, Social Genet & Dev Psychiat Res Ctr, London, England Inst Psychiat London England et & Dev Psychiat Res Ctr, London, England Penn State Univ, Ctr Dev & Hlth Genet, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 t, University Pk, PA 16802 USA Penn State Univ, Dept Biobehav Hlth, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 h, University Pk, PA 16802 USA Free Univ Amsterdam, Dept Psychol, Amsterdam, Netherlands Free Univ Amsterdam Amsterdam Netherlands ychol, Amsterdam, Netherlands Leiden Univ, Med Ctr, Dept Med Stat, Leiden, Netherlands Leiden Univ Leiden Netherlands Ctr, Dept Med Stat, Leiden, Netherlands
Titolo Testata:
GENETICAL RESEARCH
fascicolo: 2, volume: 77, anno: 2001,
pagine: 129 - 134
SICI:
0016-6723(200104)77:2<129:APARAT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUSCEPTIBILITY LOCUS; SIB-PAIRS; FAMILIES; LINKAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Slagboom, PE TNO Prevent & Hlth, Gaubius Lab, POB 2215, NL-2301 CE Leiden,Netherlands TNO Prevent & Hlth POB 2215 Leiden Netherlands NL-2301 CE ds
Citazione:
M. Beekman et al., "A powerful and rapid approach to human genome scanning using small quantities of genomic DNA", GENET RES, 77(2), 2001, pp. 129-134

Abstract

Dense maps of short-tandem-repeat polymorphisms (STRPs) have allowed genome-wide searches for genes involved in a great variety of diseases with genetic influences, including common complex diseases. Generally for this purpose, marker sets with a 10 cM spacing are genotyped in hundreds of individuals. We have performed power simulations to estimate the maximum possible inter-marker distance that still allows for sufficient power. In this paper we further report on modifications of previously published protocols, resulting in a powerful screening set containing 229 STRPs with an average spacing of 18.3 cM. A complete genome scan using our protocol requires only 80 multiplex PCR reactions which are all carried out using one set of conditionsand which do not contain overlapping marker allele sizes. The multiplex PCR reactions are grouped by sets of chromosomes, which enables on-line statistical analysis of a set of chromosomes, as sets of chromosomes are being genotyped. A genome scan following this modified protocol can be performed using a maximum amount of 2.5 mug of genomic DNA per individual, isolated from either blood or from mouth swabs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:44:50