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Titolo:
Effect of L-tryptophan supplementation on eosinophils and eotaxin in guinea pigs
Autore:
Stahl, JL; Cook, EB; Pariza, MA; Cook, ME; Graziano, FM;
Indirizzi:
Univ Wisconsin, Dept Med, Madison, WI 53706 USA Univ Wisconsin Madison WIUSA 53706 nsin, Dept Med, Madison, WI 53706 USA Univ Wisconsin, Dept Food Microbiol & Toxicol, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 biol & Toxicol, Madison, WI 53706 USA Univ Wisconsin, Dept Nutr Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Dept Nutr Sci, Madison, WI 53706 USA
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 3, volume: 226, anno: 2001,
pagine: 177 - 184
SICI:
1535-3702(200103)226:3<177:EOLSOE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYALGIA-SYNDROME; INDOLEAMINE 2,3-DIOXYGENASE; EPITHELIAL-CELLS; ASSOCIATION; INGESTION; LUNG; ACCUMULATION; TRAFFICKING; EXPRESSION; INDUCTION;
Keywords:
L-tryptophan; eosinophils; eotaxin; guinea pigs; eosinophilia myalgia syndrome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Graziano, FM Univ Wisconsin, Clin Sci Ctr H6 368, 600 Highland Ave, Madison, WI 53792 USA Univ Wisconsin 600 Highland Ave Madison WI USA 53792 3792 USA
Citazione:
J.L. Stahl et al., "Effect of L-tryptophan supplementation on eosinophils and eotaxin in guinea pigs", EXP BIOL ME, 226(3), 2001, pp. 177-184

Abstract

Eosinophilia Myalgia Syndrome is a hypereosinophilic disorder that appearsto result from the ingestion of the dietary supplement L-tryptophan by susceptible individuals. It is unclear if this disease results from tryptophan, contaminants found in tryptophan, individual predisposition (such as immune status and allergies), or some combination of effects. To evaluate effects of L-tryptophan on eosinophil migration, guinea pigs were compared with or without supplemental tryptophan (0.4 g/kg/day), with or without immune sensitization, and with or without immune challenge. Eosinophil counts were obtained from bone marrow, blood, lung, and bronchial alveolar lavage fluid(BAL), Lung cells were obtained to measure eotaxin concentrations in supernates and lysates with or without antigen and calcium ionophore challenge using direct ELISA. Skin biopsies were taken from both non-injected and antigen injection sites. The tryptophan supplemented, antigen-sensitized/antigen-challenged guinea pigs showed a significant decrease in blood eosinophils, compared to control (cellulose) supplemented antigen-sensitized/antigen-challenged guinea pigs [(0.086 +/- 0.023) x 10(6) vs (0.147 +/- 0.021) x 10(6) eosinophils/ml recovered, respectively] with a significant increase in BAL eosinophils [(0.052 +/- 0.008)x 10(6) vs (0.033 +/- 0.005) x 10(6) eosinophils/ml recovered, respectively]. Unchallenged lung cell lysates from tryptophan-supplemented guinea pigs contained significantly less eotaxin compared to cellulose-supplemented guinea pigs regardless of whether they were sensitized (0.006 +/- 0.002 vs 0.027 +/- 0.008 ng/10(6) cells, respectively). No differences were observed in skin biopsies between cellulose and tryptophan groups. These results suggest that L-tryptophan-supplemented guinea pigs have altered eotaxin regulation, a potential mechanism by which human overconsumption of tryptophan dietary supplements could lead to hypereosinophilic disorders in susceptible individuals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/12/19 alle ore 12:12:09