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Titolo:
Coumestrol antagonizes neuroendocrine actions of estrogen via the estrogenreceptor alpha
Autore:
Jacob, DA; Temple, JL; Patisaul, HB; Young, LJ; Rissman, EF;
Indirizzi:
Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA Univ Virginia Charlottesville VA USA 22904 Charlottesville, VA 22904 USA Univ Virginia, Neurosci Program, Charlottesville, VA 22904 USA Univ Virginia Charlottesville VA USA 22904 Charlottesville, VA 22904 USA Emory Univ, Ctr Behav Neurosci, Atlanta, GA 30329 USA Emory Univ Atlanta GA USA 30329 Ctr Behav Neurosci, Atlanta, GA 30329 USA
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 4, volume: 226, anno: 2001,
pagine: 301 - 306
SICI:
1535-3702(200104)226:4<301:CANAOE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA MESSENGER-RNA; MICE LACKING; GONADOTROPIN-RELEASE; PHYTOESTROGEN DIET; NEONATAL EXPOSURE; RIBONUCLEIC-ACID; KNOCKOUT MOUSE; DISRUPTED MICE; ER-ALPHA; RAT;
Keywords:
phytoestrogen; progesterone receptor; luteinizing hormone; nutrition; estrogen receptor beta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Rissman, EF Univ Virginia, Dept Biol, Gilmer Hall,POB 40-0328, Charlottesville, VA 22904 USA Univ Virginia Gilmer Hall,POB 40-0328 Charlottesville VAUSA 22904
Citazione:
D.A. Jacob et al., "Coumestrol antagonizes neuroendocrine actions of estrogen via the estrogenreceptor alpha", EXP BIOL ME, 226(4), 2001, pp. 301-306

Abstract

The phytoestrogen coumestrol has estrogenic actions on peripheral reproductive tissues. Yet in the brain this compound has both estrogenic and anti-estrogenic effects. We used estrogen receptor a knockout mice (ER alpha KO) to determine whether coumestrol has estrogenic actions in mice and also if these effects are mediated by the classic ER alpha. Female wild-type (WT) and ERaKO mice were ovariectomized and treated with estradiol (E-2), dietarycoumestrol, both, or neither compound. Ten days later the animals were sacrificed, blood was collected, and brain tissues were perfused, Fixed brainswere sectioned and immunocytochemistry was employed to quantify progesterone receptors (PR) in the medial preoptic (POA) and ventromedial nucleus of the hypothalamus (VMN). Plasma was assayed for luteinizing hormone (LH), Estrogen treatment induced PR immunoreactivity in both regions in brains of WS females, In ER alpha KO mice, lower levels of PR were induced, The stimulatory effects of E-2 on PR were attenuated in the POA by cotreatment with coumestrol, and the same trend was noted in the VMN, WT ovariectomized females treated with E-2 had low levels of LH, while LH was high in untreated females and even higher in ovariectomized females treated with coumestrol, ERalpha KO females in all treatment groups had high levels of LH, Taken together, the results show that coumestrol has anti-estrogenic actions in the brain and pituitary and that ER alpha mediates these effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:56:44