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Titolo:
5-Lipoxygenase is required for proliferation of immature cerebellar granule neurons in vitro
Autore:
Uz, T; Manev, R; Manev, H;
Indirizzi:
Univ Illinois, Dept Psychiat, Inst Psychiat, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 , Inst Psychiat, Chicago, IL 60612 USA
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 1-2, volume: 418, anno: 2001,
pagine: 15 - 22
SICI:
0014-2999(20010420)418:1-2<15:5IRFPO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT HUMAN HIPPOCAMPUS; IN-VITRO; PRIMARY CULTURES; RAT HIPPOCAMPUS; PROSTATE-CANCER; GENE-EXPRESSION; CELL-DEATH; IGF-I; NEUROGENESIS; GROWTH;
Keywords:
5-lipoxygenase; neurogenesis; antisense; AA-861; MK-886; L-655,238;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Manev, H Univ Illinois, Dept Psychiat, Inst Psychiat, 1601 W Taylor St,MC912, Chicago, IL 60612 USA Univ Illinois 1601 W Taylor St,MC912 Chicago IL USA 60612 612 USA
Citazione:
T. Uz et al., "5-Lipoxygenase is required for proliferation of immature cerebellar granule neurons in vitro", EUR J PHARM, 418(1-2), 2001, pp. 15-22

Abstract

Primary cultures of rat cerebellar granule neurons express 5-lipoxygenase,an enzyme from the inflammatory pathway of arachidonic acid. Outside the central nervous system (CNS) 5-lipoxygenase participates in cell proliferation We hypothesized that 5-lipoxygenase is needed for proliferation of immature cerebellar granule neurons. Using cultures prepared from 7-day-old rat pups, we confirmed in vitro neurogenesis by immunocytolabeling with 2-bromo-5-deoxyuridine and beta -tubulin isotype III and quantified the rate of cell proliferation by assaying [H-3]thymidine incorporation. We found that immature cerebellar granule neurons express large amounts of 5-lipoxygenase, and that treatment with a 5-lipoxygenase antisense, to reduce expression ofthis gene, decreased significantly (by 60%) the content of 5-lipoxygenase protein and effectively reduced cell proliferation. [H-3]thymidine incorporation was significantly reduced by each of the three 5-lipoxygenase inhibitors we tested: AA-861 [2-(12-hydroxydodeca-5, 10-diynyl)-3,5,6-trimethyl-1,4-benzoquinone], MK-886 (C27H33ClNO2S . Na), and L-655,238 [alpha -penyl-3-(2-quinolinylmethoxy)-benzenemethanol]. Their anti-proliferative effect wasreversible. We propose that neuronal expression of 5-lipoxygenase is crucial for neurogenesis in vitro, and possibly also in vivo. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:25:26