Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANALYSIS OF HUMAN IL-2 IL-2 RECEPTOR-BETA CHAIN INTERACTIONS - MONOCLONAL-ANTIBODY H2-8 AND NEW IL-2 MUTANTS DEFINE THE CRITICAL ROLE OF ALPHA-HELIX-A OF IL-2
Autore:
ECKENBERG R; XU D; MOREAU JL; BOSSUS M; MAZIE JC; TARTAR A; LIU XY; ALZARI PM; BERTOGLIO J; THEZE J;
Indirizzi:
INST PASTEUR,25 RUE DR ROUX F-75015 PARIS FRANCE INST PASTEUR F-75015 PARIS FRANCE FAC PHARM PARIS 11,INSERM CJF 93 01 CHATENAY MALABR FRANCE INST PASTEUR F-59019 LILLE FRANCE SHANGHAI INST BIOCHEM SHANGHAI PEOPLES R CHINA
Titolo Testata:
Cytokine
fascicolo: 7, volume: 9, anno: 1997,
pagine: 488 - 498
SICI:
1043-4666(1997)9:7<488:AOHIIR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN INTERLEUKIN-2 RECEPTOR; HIGH-AFFINITY; GAMMA-CHAIN; MOUSE INTERLEUKIN-2; CELL-LINE; EXPRESSION; BINDING; P55; IDENTIFICATION; RESIDUES;
Keywords:
IL-2 STRUCTURE; FUNCTION; IL-2 RECEPTORS; IL-2 MUTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
R. Eckenberg et al., "ANALYSIS OF HUMAN IL-2 IL-2 RECEPTOR-BETA CHAIN INTERACTIONS - MONOCLONAL-ANTIBODY H2-8 AND NEW IL-2 MUTANTS DEFINE THE CRITICAL ROLE OF ALPHA-HELIX-A OF IL-2", Cytokine, 9(7), 1997, pp. 488-498

Abstract

Interleukin 2 (IL-2) interacts with a receptor (IL-2R) composed of three subunits (IL-2R alpha, IL-2R beta and IL-2R gamma), IL-2R beta plays a critical role in signal transduction. An anti-human IL-2 mAb (H2-8) produced after immunization with peptide 1-30 of IL-2 was found to recognize the region occupied by Asp20, at the exposed interface between cr-helices A and C, Muteins at position 17 and 20 are not recognized by mAb H2-8. mAb H2-8 specifically inhibits the IL-2 proliferation of TS1 beta cells which are dependent on the expression of human IL-2R beta chain for IL-2 proliferation, Substitution at internal position Leu17 demonstrates that this position is essential for IL-2 binding andIL-2 bioactivity. New IL-2 mutants at position Asp20 have been analysed, Substitutions Asp --> Asn, Asp --> Lys, Asp --> Leu, show a correlation between diminished affinity for IL-2 receptor and reduced bioactivity measured on TS1 beta cells, Mutein Asp --> Arg lose affinity forIL-2R and bioactivity simultaneously. Furthermore, during the course of the study we have found that mutein Asp20 --> Leu is an IL-2 antagonist, The biological effects of mAb H2-8 and the properties of new mutants at positions 17 and 20 demonstrate that this region of or helix-Ais involved in IL-2-IL-2R beta interactions. (C) 1997 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 10:17:05