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Titolo:
Prostasome-like granules from the PC-3 prostate cancer cell line increase the motility of washed human spermatozoa and adhere to the sperm
Autore:
Wang, J; Lundqvist, M; Carlsson, L; Nilsson, O; Lundkvist, O; Ronquist, G;
Indirizzi:
Univ Uppsala Hosp, Dept Med Sci Clin Chem, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 n Chem, S-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Womens & Childrens Hlth, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 s Hlth, S-75185 Uppsala, Sweden Ctr Med Biol, Dept Med Cellbiol, S-75185 Uppsala, Sweden Ctr Med Biol Uppsala Sweden S-75185 ed Cellbiol, S-75185 Uppsala, Sweden Quighai Med Coll Hosp, Dept Urol, Xining 810001, Peoples R China Quighai Med Coll Hosp Xining Peoples R China 810001 001, Peoples R China
Titolo Testata:
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
fascicolo: 1, volume: 96, anno: 2001,
pagine: 88 - 97
SICI:
0301-2115(200105)96:1<88:PGFTPP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIPEPTIDYL-PEPTIDASE-IV; SEMEN ANALYSIS SYSTEMS; HUMAN SEMINAL PLASMA; SWIM-UP MEDIUM; COLLAGEN; INVOLVEMENT; FERTILITY; INCLUSION; DISTINCT; VESICLES;
Keywords:
anti-prostasome antibody; immunocytochemistry; PC-3 cells; prostasomes; sperm motility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ronquist, G Univ Uppsala Hosp, Dept Med Sci Clin Chem, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 185 Uppsala, Sweden
Citazione:
J. Wang et al., "Prostasome-like granules from the PC-3 prostate cancer cell line increase the motility of washed human spermatozoa and adhere to the sperm", EUR J OB GY, 96(1), 2001, pp. 88-97

Abstract

Objective: Prostasome-like granules are present in the PC-3 prostate cancer cells. Since the seminal prostasomes are able to promote the forward motility of human spermatozoa. we conducted a study to determine whether PC-3 prostasomes exerted effects similar to those of seminal prostasomes on buffer-washed spermatozoa from normospermic semen samples. Study design: We usedcomputer-assisted sperm analysis (CASA) and immunostaining of prostasomes to find out where these granules are located on the spermatozoa. Results: Addition of PC-3 prostasomes increased the proportion of motile spermatozoa from 12-15% to 50-70% (p <0.001). The optimal protein concentration of these prostasomes was 0.1 mg/ml. Heat treatment of PC-3 prostasomes did not decrease their motility-promoting effect. Immunostaining with anti-prostasome monoclonal antibody (mAb78) revealed that the PC-3 prostasomes and seminal prostasomes adhered to the sperm cells. The staining, which occurred all over the spermatozoa. was intense on the mid-pieces and weaker on the sperm heads. Herewith, some prostasome component may activate the spermatic mitochondrial function, thus increasing sperm motility. Conclusion: it is concluded that PC-3 prostasomes bear a functional resemblance to seminal prostasomes as regards sperm motility promotion. <(c)> 2001 Elsevier Science IrelandLtd. All rights reserved.

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Documento generato il 05/12/20 alle ore 13:50:59