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Titolo:
Sindbis viral-mediated expression of Ca2+-permeable AMPA receptors at hippocampal CA1 synapses and induction of NMDA receptor-independent long-term potentiation
Autore:
Okada, T; Yamada, N; Kakegawa, W; Tsuzuki, K; Kawamura, M; Nawa, H; Iino, M; Ozawa, S;
Indirizzi:
Gunma Univ, Sch Med, Dept Physiol, Gunma 3718511, Japan Gunma Univ GunmaJapan 3718511 h Med, Dept Physiol, Gunma 3718511, Japan Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, Japan Japan Sci & Technol Corp Kawaguchi Saitama Japan 3320012 a 3320012, Japan Niigata Univ, Brain Res Inst, Dept Mol Neurobiol, Niigata 9518585, Japan Niigata Univ Niigata Japan 9518585 Mol Neurobiol, Niigata 9518585, Japan
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 8, volume: 13, anno: 2001,
pagine: 1635 - 1643
SICI:
0953-816X(200104)13:8<1635:SVEOCA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TRANSFER; NEURONS; CHANNELS; CALCIUM; VIRUS; SLICE; RECTIFICATION; ACTIVATION; DENDRITES; CULTURES;
Keywords:
green fluorescent protein; GluR2; rat; slice culture; viral vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ozawa, S Gunma Univ, Sch Med, Dept Physiol, 3-39-22 Showa Machi, Gunma 3718511, Japan Gunma Univ 3-39-22 Showa Machi Gunma Japan 3718511 718511, Japan
Citazione:
T. Okada et al., "Sindbis viral-mediated expression of Ca2+-permeable AMPA receptors at hippocampal CA1 synapses and induction of NMDA receptor-independent long-term potentiation", EUR J NEURO, 13(8), 2001, pp. 1635-1643

Abstract

Gene manipulation in order to artificially express a particular gene in neurons in the central nervous system is a powerful tool for the analysis of brain function. Sindbis viral vectors have been developed to express high levels of foreign genes in postmitotic brain neurons with little transfection of glial cells. In this study, we expressed the gene encoding the unedited GluR2 (GluR-B) subunit of the AMPA-type glutamate receptor that forms inwardly rectifying and Ca2+-permeable channels, in rat CA1 hippocampal neurons in slice cultures using Sindbis viral vectors. The pyramidal cell layer of the CA1 region was injected with recombinant Sindbis viruses encoding both enhanced green fluorescent protein (GFP) and unedited GluR2. The GFP fluorescence from CA1 neurons could be detected as early as 6 h and reached a maximal level about 48 h postinfection. The inwardly rectifying and Ca2+-permeable AMPA receptors were expressed in most CA1 pyramidal cells expressingGFP. These AMPA receptors expressed by gene transfer were involved in fastexcitatory neurotransmission elicited by electrical stimulation of the Schaffer collaterals in the stratum radiatum. Tetanic stimulation of Schaffer collaterals induced NMDA receptor-independent, long-term potentiation due to Ca2+ influx through the newly expressed AMPA receptors in the area densely stained with GFP. Thus, the combined use of Sindbis viral vectors with the GFP reporter allowed physiological examination of the roles of a specificgene product in synaptic function in well-characterized brain neurons.

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Documento generato il 19/01/20 alle ore 09:01:57