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Titolo:
Laminar specific alterations of thalamocortical projections in organotypiccultures following layer 4 disruption in ferret somatosensory cortex
Autore:
Palmer, SL; Noctor, SC; Jablonska, B; Juliano, SL;
Indirizzi:
Uniformed Serv Univ Hlth Sci, Dept Anat Physiol & Genet, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci, Program Neurosci, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 8, volume: 13, anno: 2001,
pagine: 1559 - 1571
SICI:
0953-816X(200104)13:8<1559:LSAOTP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT VISUAL-CORTEX; LATERAL GENICULATE-NUCLEUS; SLICE CULTURES; CONNECTIONS; NEOCORTEX; METHYLAZOXYMETHANOL; BRAIN; CELLS; INNERVATION; MIGRATION;
Keywords:
cerebral cortex; cortical dysplasia; development; in vitro; MAM; migration disorder;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Juliano, SL Uniformed Serv Univ Hlth Sci, Dept Anat Physiol & Genet, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 20814 USA
Citazione:
S.L. Palmer et al., "Laminar specific alterations of thalamocortical projections in organotypiccultures following layer 4 disruption in ferret somatosensory cortex", EUR J NEURO, 13(8), 2001, pp. 1559-1571

Abstract

The developing neocortex influences the growth of thalamocortical projections. Layer 4 in particular receives the majority of input from the thalamusand is important in instructing thalamic afferents to terminate. Previous in vivo experiments demonstrated that disruption of layer 4 during corticogenesis in ferret somatosensory cortex by application of methylazoxy methanol acetate (MAM) prevents proper termination of thalamic afferents in appropriate cortical regions. To further explore the role of layer 4 in thalamocortical development, we prepared organotypic cocultures consisting of normalgestational day 0 (P0) ferret thalamus paired with normal, embryonic day 33 (E33), or E38 MAM-treated cortex obtained from ferrets at either P0 or P7. Injection of MAM on E33 disrupts layer 4 formation, whereas similar injections on E38 interfere with layer 2 formation. The cocultures grew togetherfor a number of days, then discrete injections of either fluorescent dextrans or 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) were made into the thalamic piece. The labeled thalamic afferents that grew into the cortical slice were analysed and the sites of their terminations quantified after 3, 5, or 7-10 days in culture (DIC). Our results varied somewhat with the amount of time in culture, but the preponderance of thalamic fibers in normal cortex terminated in layer 4, whereas their counterparts in E33 MAM-treated cortex grew beyond the cortical plate and many fibers terminated inappropriately within lower cortical layers or white matter. Terminal distribution of thalamic fibers in E38 MAM-treated cortex looked similar to normal. These results demonstrate that the cells of layer 4 provide thalamic afferents with important positional and termination cues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:45:25