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Titolo:
Success and failure with phthalate buffers in capillary tone electrophoresis
Autore:
Bocek, P; Gebauer, P; Beckers, JL;
Indirizzi:
Acad Sci Czech Republ, Inst Analyt Chem, CZ-61142 Brno, Czech Republic Acad Sci Czech Republ Brno Czech Republic CZ-61142 Brno, Czech Republic Eindhoven Univ Technol, Dept Chem SP, NL-5600 MB Eindhoven, Netherlands Eindhoven Univ Technol Eindhoven Netherlands NL-5600 MB ven, Netherlands
Titolo Testata:
ELECTROPHORESIS
fascicolo: 6, volume: 22, anno: 2001,
pagine: 1106 - 1111
SICI:
0173-0835(200104)22:6<1106:SAFWPB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIRECT PHOTOMETRIC DETECTION; INDIRECT UV DETECTION; PREDICTING PEAK SYMMETRY; ZONE-ELECTROPHORESIS; ORGANIC-ACIDS; BACKGROUND ELECTROLYTES; LIQUID-CHROMATOGRAPHY; ION CHROMATOGRAPHY; METHYLMALONIC ACID; PHOSPHATE BUFFER;
Keywords:
phthalate buffer; system peaks; electromigration dispersion; multivalent buffers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Bocek, P Acad Sci Czech Republ, Inst Analyt Chem, Veveri 97, CZ-61142 Brno, Czech Republic Acad Sci Czech Republ Veveri 97 Brno Czech Republic CZ-61142 lic
Citazione:
P. Bocek et al., "Success and failure with phthalate buffers in capillary tone electrophoresis", ELECTROPHOR, 22(6), 2001, pp. 1106-1111

Abstract

Phthalate buffers are currently used in capillary electrophoresis as robust electrolyte systems for indirect detection. This contribution demonstrates that these buffers show regularly not only successful regions of mobilities of analytes (sample window) but also regions of failure where the migration of analytes is strongly deteriorated due to the presence of a system zone. System zones in phthalate buffers may be easily detected by UV detection and manifest themselves as peaks or dips. Peak shape diagrams are advantageously used for the prediction of the migration behavior of system zones in phthalate background electrolyte (BGE) systems at various pH. It is shownthat the mobility of the system zone varies strongly with pH, is practically zero at pH values below 4 and above 7, and shows a maximum at pH 5. Thus, the system peak may coincide either with the peaks of various analytes orwith the electroosmotic flow (EOF) peak. Experiments are given showing theeffects of such coincidences as, e.g., zigzag detection patterns, double EOF peaks, and/or unusually broad peaks/dips. The message of this contribution is to show how to understand the electrophoretic properties of phthalateBGEs that, regardless of possible failure regions, may be successfully used in the analytical practice of capillary zone electrophoresis (CZE).

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Documento generato il 01/04/20 alle ore 17:33:50