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Titolo:
Electrochemical monitoring of plant stress responses
Autore:
Kinpara, T; Murakami, Y; Yokoyama, K; Tamiya, E;
Indirizzi:
Japan Adv Inst Sci & Technol, Tatsunokuchi, Ishikawa 9231292, Japan Japan Adv Inst Sci & Technol Tatsunokuchi Ishikawa Japan 9231292 2, Japan
Titolo Testata:
ELECTROANALYSIS
fascicolo: 6, volume: 13, anno: 2001,
pagine: 451 - 456
SICI:
1040-0397(200104)13:6<451:EMOPSR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SUSPENSION CULTURES; LEUKEMIA RBL-1 CELLS; PLASMA-MEMBRANE; OXIDATIVE BURST; SALICYLIC-ACID; RESISTANCE RESPONSE; NADH OXIDASE; TOBACCO; LOCALIZATION; ACCUMULATION;
Keywords:
plant; stress response; electrochemical reduction activity; electron acceptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Tamiya, E Japan Adv Inst Sci & Technol, 1-1 Asahidai, Tatsunokuchi, Ishikawa 9231292, Japan Japan Adv Inst Sci & Technol 1-1 Asahidai Tatsunokuchi Ishikawa Japan 9231292
Citazione:
T. Kinpara et al., "Electrochemical monitoring of plant stress responses", ELECTROANAL, 13(6), 2001, pp. 451-456

Abstract

Intact cultured cells of Nicotiana tabacum BY2 cells exhibited the electrochemical reduction of I, l-benzoquinone (BQ), 2,6-dichloroindophenol (DCIP), hexacyanoferrate (ferricyanide). This was detected by amperometry with fixed potential (400 mV vs. Ag/AgCl) using a film-coated phi 1.6 mm platinum disk electrode as a working electrode. The electrochemical activities of BQ, DCIP, and ferricyanide mixed with BY2 cell were changed after the addition of various stress compounds such as elicitors, plant hormones, metabolic and electron transport inhibitors. Different stress compounds gave di different electrochemical patterns probably due to the different positions of electron accepters affected by these bio-materials. In addition, we observed the generation of hydrogen peroxide in BY2 cells treated with various stress compounds, The pattern of chemiluminescence is useful for the determination of the intensity of plant stress responses. High accumulation of hydrogen peroxide was observed in the BY2 cells treated with elicitors (salicylic acid and ethylene glycol chitin) and plant hormone (2,3-D). A relationship between electrochemical patterns and stress compounds were also discussed.

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Documento generato il 31/03/20 alle ore 09:47:02