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Titolo:
Differentiation stages of eosinophils characterized by hyaluronic acid binding via CD44 and responsiveness to stimuli
Autore:
Watanabe, Y; Hashizume, M; Kataoka, S; Hamaguchi, E; Morimoto, N; Tsuru, S; Katoh, S; Miyake, K; Matsushima, K; Tominaga, M; Kurashige, T; Fujimoto, S; Kincade, PW; Tominaga, A;
Indirizzi:
Kochi Med Sch, Dept Med Biol, Nankoku, Kochi 7838505, Japan Kochi Med SchNankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan Kochi Med Sch, Dept Pediat, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan Kochi Med Sch, Med Res Ctr, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan Kochi Med Sch, Radioisotope Ctr, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan Kochi Med Sch, Clin Lab, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan Kochi Med Sch, Dept Immunol, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan Miyazaki Med Coll, Dept Internal Med 3, Miyazaki 88916, Japan Miyazaki MedColl Miyazaki Japan 88916 rnal Med 3, Miyazaki 88916, Japan Saga Med Sch, Dept Immunol, Saga, Japan Saga Med Sch Saga JapanSaga Med Sch, Dept Immunol, Saga, Japan Univ Tokyo, Sch Med, Dept Prevent Med, Tokyo 113, Japan Univ Tokyo TokyoJapan 113 , Sch Med, Dept Prevent Med, Tokyo 113, Japan Oklahoma Med Res Fdn, Immunobiol & Canc Program, Oklahoma City, OK 73104 USA Oklahoma Med Res Fdn Oklahoma City OK USA 73104 lahoma City, OK 73104 USA
Titolo Testata:
DNA AND CELL BIOLOGY
fascicolo: 4, volume: 20, anno: 2001,
pagine: 189 - 202
SICI:
1044-5498(200104)20:4<189:DSOECB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR ENDOTHELIAL-CELLS; NERVE GROWTH-FACTOR; TRANSGENIC MICE; B-CELLS; LYMPHO-HEMATOPOIESIS; TISSUE EOSINOPHILIA; MONOCLONAL-ANTIBODY; IL-5 RECEPTOR; BONE-MARROW; T-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Tominaga, A Kochi Med Sch, Dept Med Biol, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 ochi 7838505, Japan
Citazione:
Y. Watanabe et al., "Differentiation stages of eosinophils characterized by hyaluronic acid binding via CD44 and responsiveness to stimuli", DNA CELL B, 20(4), 2001, pp. 189-202

Abstract

To characterize interleukin (IL)-5-induced eosinophils, we examined the expression of CD44, very late antigen (VLA)-4 and the IL-5 receptor alpha chain, as well as the levels of eosinophil peroxidase and the generation of superoxide. Eosinophils were prepared from IL-5-transgenic mice, then characterized using electron microscopy to determine their responses to stimuli. Whereas CD44 densities remained almost constant, the level of VLA-4 increased in parallel with eosinophil maturation. Although a subset of IL-5-inducedeosinophils with high side scatter recovered from bone marrow and rare ones found in blood recognized hyaluronic acid (HA), most did not have this property, Bone marrow eosinophils with high side scatter and lower density contained eosinophil peroxidase, not only in granules, but also in membranousstructures for 30% of this population. This population developed HA-binding ability in response to IL-3, IL-4, IL-5, granulocyte-macrophage colony-stimulating factor, macrophage inflammatory protein (MIP)-2, monocyte chemotactic protein (MCP)-1, eotaxin, nerve growth factor (NGF), and opsonized zymosan (OZ), Peripheral blood eosinophils acquired HA-binding ability in response to the same stimuli, but their responses were less than those of bone marrow eosinophils with high levels of side scatter. However, splenic eosinophils did not respond to these stimuli. Although peripheral blood eosinophils did not proliferate when stimulated by IL-5, these were the only cells that released eosinophil peroxidase in response to IL-4, MIP-2, MCP-1, eotaxin, NGF, and OZ. With the exception of a subset of bone marrow eosinophils? the ability to acquire HA binding, but not the ability to generate superoxide, correlated with eosinophil peroxidase activity and major basic protein accumulation in the granules of maturing cells.

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Documento generato il 13/07/20 alle ore 17:12:11