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Titolo:
In vivo functional analysis of ezrin during mouse blastocyst formation
Autore:
Dard, N; Louvet, S; Santa-Maria, A; Aghion, J; Martin, M; Mangeat, P; Maro, B;
Indirizzi:
Univ Paris 06, UMR 7622 CNRS, Lab Biol Cellulaire Dev, F-75252 Paris 05, France Univ Paris 06 Paris France 05 l Cellulaire Dev, F-75252 Paris 05, France Univ Montpellier 2, CNRS UMR 5539, F-34095 Montpellier, France Univ Montpellier 2 Montpellier France F-34095 -34095 Montpellier, France
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 233, anno: 2001,
pagine: 161 - 173
SICI:
0012-1606(20010501)233:1<161:IVFAOE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL EXTENSION ACTIVITY; AMINO-TERMINAL DOMAIN; ERM FAMILY MEMBERS; PLASMA-MEMBRANE; MICROVILLAR BREAKDOWN; SURFACE POLARITY; ACTIN-BINDING; PROTEIN; EMBRYO; LOCALIZATION;
Keywords:
ERM proteins; epithelium differentiation; embryo; trophectoderm; cavitation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Maro, B Univ Paris 06, UMR 7622 CNRS, Lab Biol Cellulaire Dev, 9 Quai St Bernard, F-75252 Paris 05, France Univ Paris 06 9 Quai St Bernard Paris France 05 Paris 05, France
Citazione:
N. Dard et al., "In vivo functional analysis of ezrin during mouse blastocyst formation", DEVELOP BIO, 233(1), 2001, pp. 161-173

Abstract

During mouse blastocyst formation, a layer of outer cells differentiates in less than 48 h into a functional epithelium (the trophectoderm). Ezrin, an actin-binding structural component of microvilli in epithelial cells, is also involved in signal transduction and ionic pump control. In the mouse embryo, ezrin becomes restricted to the apical cortex of all blastomeres at compaction and of outer cells at later stages. Here we investigated the function of ezrin in living embryos during epithelial differentiation using mutant forms of ezrin tagged with green fluorescent protein (GFP). GFP-taggedwild-type ezrin (Ez/GFPc) behaved like endogenous ezrin and did not interfere with development. Deletion of the last 53 amino acids (Delta 53/GFP) changed the localization of ezrin: after compaction, Delta 53/GFP remained associated with the apical and basolateral cortex in all blastomeres, and itsexpression slightly disturbed the cavitation process. Finally, full-lengthezrin with GFP inserted at position 234 (Ez/GFPi) was localized all aroundthe cortex throughout development, although it was concentrated at tile apical pole after compaction. In embryos expressing Ez/GFPi, the duration of the 16-cell stage was reduced, while the onset of cavitation was delayed. Moreover, cavitation was abnormal, and the blastocoele was small and retracted almost completely several times as if there were major leakages of blastocoelic fluid. Our results suggest that, in addition to its role in microvilli organization, ezrin is involved in the formation of a functional epithelium through a still unknown mechanism. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:12:26