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Titolo:
Misexpression of Fgf-4 in the chick limb inhibits myogenesis by down-regulating Frek expression
Autore:
Edom-Vovard, F; Bonnin, MA; Duprez, D;
Indirizzi:
Inst Embryol Cellulaire & Mol, CNRS FRE 2160, F-94736 Nogent Sur Marne, France Inst Embryol Cellulaire & Mol Nogent Sur Marne France F-94736 ne, France Coll France, F-94736 Nogent Sur Marne, France Coll France Nogent Sur Marne France F-94736 736 Nogent Sur Marne, France
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 233, anno: 2001,
pagine: 56 - 71
SICI:
0012-1606(20010501)233:1<56:MOFITC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; SKELETAL-MUSCLE DEVELOPMENT; FACTOR RECEPTOR FAMILY; TERMINAL DIFFERENTIATION; CELL-DIFFERENTIATION; PAX-3 EXPRESSION; FIBER-TYPE; MOUSE; GENE; PROLIFERATION;
Keywords:
Fgf-4; MyoD; chick; limb bud; myogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: Duprez, D Inst Embryol Cellulaire & Mol, CNRS FRE 2160, 49 Bis,Ave Belle Gabrielle, F-94736 Nogent Sur Marne, France Inst Embryol Cellulaire & Mol 49Bis,Ave Belle Gabrielle Nogent Sur Marne France F-94736
Citazione:
F. Edom-Vovard et al., "Misexpression of Fgf-4 in the chick limb inhibits myogenesis by down-regulating Frek expression", DEVELOP BIO, 233(1), 2001, pp. 56-71

Abstract

Skeletal muscle development involves an initial period of myoblast replication followed by a phase in which some myoblasts continue to proliferate while others undergo terminal differentiation. The latter process involves the permanent cessation of DNA synthesis, activation of muscle-specific gene expression, and fusion of single cells to generate multinucleated muscle fibres. The in vivo signals regulating the progression through all these steps remain unknown. Fibroblast growth factors (Fgfs) and Fgf receptors comprise a large family whose members have been shown to play multiple roles in the development of skeletal muscle in vitro. Exogenously applied Fgfs are able to stimulate proliferation and suppress myogenic differentiation in cellculture. We sought to determine the role played by Fgf-4 during limb myogenesis in vivo. Fgf-4 transcripts are located at both extremities of myotubes whereas the mRNAs of one of the Fgf receptors, Frek, are detected in mononucleated proliferating myoblasts surrounding the multinucleated fibres. Overexpression of mouse Fgf-4 (mFgf-4) using a replication-competent retrovirus, RCAS, leads to a down-regulation of muscle markers followed by an inhibition of terminal differentiation in limb muscles. Using quail/chick transplantations we were able to follow the muscle cells and found a dramatic decrease in their number after exposure to mFgf-4. Interestingly ectopic mFgf-4 down-regulates Frek transcripts in limb muscle areas. We conclude that overexpression of mFgf-4 inhibits myoblast proliferation, probably by down-regulating Frek mRNAs. This suggests a role for Fgf-4, located at the extremities of the myotubes, where it could be responsible for the absence of FrekmRNA in the muscle fibre. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 02:59:46