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Titolo:
The role of presenilin 1 during somite segmentation
Autore:
Koizumi, K; Nakajima, M; Yuasa, S; Saga, Y; Sakai, T; Kuriyama, T; Shirasawa, T; Koseki, H;
Indirizzi:
Chiba Univ, Grad Sch Med, Dept Mol Embryol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 ol Embryol, Chuo Ku, Chiba 2608670, Japan Chiba Univ, Grad Sch Med, Dept Chest Med, Chiba 2608670, Japan Chiba UnivChiba Japan 2608670 Med, Dept Chest Med, Chiba 2608670, Japan Tokyo Metropolitan Inst Gerontol, Dept Mol Genet, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 okyo 1730015, Japan Chiba Univ, Grad Sch Med, Dept Anat & Dev Biol, Chiba 2608670, Japan ChibaUniv Chiba Japan 2608670 ept Anat & Dev Biol, Chiba 2608670, Japan Natl Inst Hlth Sci, Cellular & Mol Toxicol Div, Setagaya Ku, Tokyo 158, Japan Natl Inst Hlth Sci Tokyo Japan 158 ol Div, Setagaya Ku, Tokyo 158, Japan
Titolo Testata:
DEVELOPMENT
fascicolo: 8, volume: 128, anno: 2001,
pagine: 1391 - 1402
SICI:
0950-1991(200104)128:8<1391:TROP1D>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYLOID PRECURSOR PROTEIN; LUNATIC FRINGE; MOUSE EMBRYO; DELTA-HOMOLOG; PARAXIAL MESODERM; NERVOUS-SYSTEM; NOTCH; GENE; EXPRESSION; DROSOPHILA;
Keywords:
somite; presenilin 1; Mesp2; Notch1; lunatic fringe; Dll1; segmentation; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Koseki, H Chiba Univ, Grad Sch Med, Dept Mol Embryol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 , Chuo Ku, Chiba 2608670, Japan
Citazione:
K. Koizumi et al., "The role of presenilin 1 during somite segmentation", DEVELOPMENT, 128(8), 2001, pp. 1391-1402

Abstract

The Notch signalling pathway plays essential roles during the specification of the rostral and caudal somite halves and subsequent segmentation of the paraxial mesoderm, We have re-investigated the role of presenilin 1 (Ps1;encoded by Psen1) during segmentation using newly generated alleles of thePsen1 mutation. In Psen1-deficient mice, proteolytic activation of Notch1 was significantly affected and the expression of several genes involved in the Notch signalling pathway was altered, including Delta-like3, Hes5, lunatic fringe (Lfng) and Mesp2. Thus, Ps1-dependent activation of the Notch pathway is essential for caudal half somite development. We observed defects in Notch signalling in both the caudal and rostral region of the presomiticmesoderm. In the caudal presomitic mesoderm, Ps1 was involved in maintaining the amplitude of cyclic activation of the Notch pathway, as represented by significant reduction of Lfng expression in Psen1-deficient mice. In therostral presomitic mesoderm, rapid downregulation of the Mesp2 expression in the presumptive caudal half somite depends on Ps1 and is a prerequisite for caudal somite half specification. Chimaera analysis between Psen1-deficient and wild-type cells revealed that condensation of the wild-type cells in the caudal half somite was concordant with the formation of segment boundaries, while mutant and wild-type cells intermingled in the presomitic mesoderm. This implies that periodic activation of the Notch pathway in the presomitic mesoderm is still latent to segregate the presumptive rostral and caudal somite. A transient episode of Mesp2 expression might be needed for Notch activation by Ps1 to confer rostral or caudal properties. In summary,we propose that Ps1 is involved in the functional manifestation of the segmentation clock in the presomitic mesoderm.

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Documento generato il 22/01/20 alle ore 07:02:02