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Titolo:
Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction
Autore:
Kupferschmidt, O; Kruger, D; Held, TK; Ellerbrok, H; Siegert, W; Janitschke, K;
Indirizzi:
Robert Koch Inst, D-13353 Berlin, Germany Robert Koch Inst Berlin Germany D-13353 ch Inst, D-13353 Berlin, Germany Humboldt Univ, Klin Innere Med Schwerpunkt Hamatol, D-13353 Berlin, Germany Humboldt Univ Berlin Germany D-13353 kt Hamatol, D-13353 Berlin, Germany
Titolo Testata:
CLINICAL MICROBIOLOGY AND INFECTION
fascicolo: 3, volume: 7, anno: 2001,
pagine: 120 - 124
SICI:
1198-743X(200103)7:3<120:QDOTGD>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARROW TRANSPLANT RECIPIENTS; REAL-TIME PCR; AIDS PATIENTS; COMPETITIVE PCR; CONGENITAL TOXOPLASMOSIS; CEREBRAL TOXOPLASMOSIS; CEREBROSPINAL-FLUID; PRENATAL-DIAGNOSIS; AMPLIFICATION; ENCEPHALITIS;
Keywords:
TaqMan polymerase chain reaction; r-DNA; Toxoplasma; toxoplasmosis; bone marrow transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kruger, D Robert Koch Inst, Nordufer 20, D-13353 Berlin, Germany Robert Koch Inst Nordufer 20 Berlin Germany D-13353 in, Germany
Citazione:
O. Kupferschmidt et al., "Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction", CL MICRO IN, 7(3), 2001, pp. 120-124

Abstract

Objective A new quantitative polymerase chain reaction (real-time PCR) wasdesigned to detect Toxoplasma DNA in human body fluid samples. Methods Real-time fluorescence detection of amplification product formation on the basis of the TaqMan-System was established with Toxoplasma 18S rDNA as a target gene. Results The method provides a high sensitivity comparable to conventional nested PCR procedures and generates quantitative data when detecting toxoplasmic DNA in human blood, cerebrospinal or amniotic fluid. Moreover, data were obtained investigating blood samples from an immunocompromised patient with reactivated toxoplasmosis after allogeneic bone marrow transplantation. monitoring the therapeutic effect. Conclusions The potential application of this method to detect Toxoplasma,ln DNA in body fluids and to follow the development of parasitemia under therapy could be demonstrated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 03:55:33