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Titolo:
Heterogeneity of DNA methylation status analyzed by bisulfite-PCR-SSCP andcorrelation with clinico-pathological characteristics in colorectal cancer
Autore:
Maekawa, M; Sugano, K; Ushiama, M; Fukayama, N; Nomoto, K; Kashiwabara, H; Fujita, S; Kakizoe, T;
Indirizzi:
Natl Canc Ctr Hosp, Clin Lab, Tokyo, Japan Natl Canc Ctr Hosp Tokyo Japan tl Canc Ctr Hosp, Clin Lab, Tokyo, Japan
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 2, volume: 39, anno: 2001,
pagine: 121 - 128
SICI:
1434-6621(200102)39:2<121:HODMSA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND CONFORMATION POLYMORPHISM; CPG ISLAND METHYLATION; MICROSATELLITE INSTABILITY; HMLH1 PROMOTER; COLON-CANCER; CELL-LINES; HYPERMETHYLATION; GENES; ASSAY; INACTIVATION;
Keywords:
methylation; bisulfite-PCR-SSCP; colorectal cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Maekawa, M Hamamatsu Univ, Sch Med, Dept Lab Med, Handa Cho 3600, Hamamatsu, Shizuoka4313192, Japan Hamamatsu Univ Handa Cho 3600 Hamamatsu Shizuoka Japan 4313192
Citazione:
M. Maekawa et al., "Heterogeneity of DNA methylation status analyzed by bisulfite-PCR-SSCP andcorrelation with clinico-pathological characteristics in colorectal cancer", CLIN CH L M, 39(2), 2001, pp. 121-128

Abstract

Aberrant DNA methylation has been identified as an important mechanism forinactivation of tumor suppressor genes and mismatch repair genes during carcinogenesis. We used bisulfite treatment and the PCR-single strand conformation polymorphism (SSCP) (BiPS) technique to analyze methylation status ofthe promoter regions of the hMLH1, p16, and HIC1 genes in several cancer cell lines and colorectal cancer tissues. The methylation of the hMLH1, p16 and HIC1 genes was observed in 2, 8, and13 of 13 cancer cell lines, respectively. The SSCP for p16 and HIC1 in each of the methylation-positive cell lines were similar, indicating relative homogeneity of methylation status and complete methylation in the cell lines. Methylation was observed in 8, 5, and 21 of 25 colorectal cancer tissuesfor the hMLH1, p16, and HIC1 genes, respectively. The methylated bands revealed by BiPS analysis of the hMLH1 gene were homogeneous, whereas those ofthe p16 and HIC1 genes were different in each case. The methylation of thepromoter region of the HIC1 gene in colorectal cancer was observed most frequently and could serve as a sensitive marker for colorectal cancer. Methylation status of the hMLH1 and p16 gene promoters was correlated with microsatellite instability status, tumor location, and differentiation but not with K-ras mutation or allelic loss of p53.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:39:57