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Titolo:
Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis
Autore:
Ferrando-May, E; Cordes, V; Biller-Ckovric, I; Mirkovic, J; Gorlich, D; Nicotera, P;
Indirizzi:
Univ Konstanz, Dept Biol, Chair Mol Toxicol, D-78457 Constance, Germany Univ Konstanz Constance Germany D-78457 icol, D-78457 Constance, Germany Karolinska Inst, Med Nobel Inst, Dept Cellular & Mol Biol, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 Biol, S-17177 Stockholm, Sweden German Canc Res Ctr, Div Cell Biol, D-69120 Heidelberg, Germany German Canc Res Ctr Heidelberg Germany D-69120 69120 Heidelberg, Germany Univ Heidelberg, Zentrum Mol Biol, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany Univ Leicester, MRC, Toxicol Unit, Leicester LE1 9HN, Leics, England Univ Leicester Leicester Leics England LE1 9HN er LE1 9HN, Leics, England
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 5, volume: 8, anno: 2001,
pagine: 495 - 505
SICI:
1350-9047(200105)8:5<495:CMNCBN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORE COMPLEX PROTEIN; MESSENGER-RNA EXPORT; IMPORT FACTOR P97; INTRANUCLEAR FILAMENTS; CHROMATIN CONDENSATION; XENOPUS-LAEVIS; BINDING DOMAIN; CELL-DEATH; NUP153; RAN;
Keywords:
caspase; nuclear pore complex; nuclear import; mRNA export; microinjection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Nicotera, P Univ Leicester, MRC, Toxicol Unit, Hodgkin Bldg,Lancaster Rd, Leicester LE1 9HN, Leics, England Univ Leicester Hodgkin Bldg,Lancaster Rd Leicester Leics England LE1 9HN
Citazione:
E. Ferrando-May et al., "Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis", CELL DEAT D, 8(5), 2001, pp. 495-505

Abstract

In eukaryotic cells, both soluble transport factors and components of the nuclear pore complex mediate protein and RNA trafficking between the nucleus and the cytoplasm, Here, we investigated whether caspases, the major execution system in apoptosis, target the nuclear pore or components of the nuclear transport machinery. Four nucleoporins, Nup153, RanBP2, Nup214 and Tprare cleaved by caspases during apoptosis, In contrast, the nuclear transport factors, Ran, importin alpha and importin beta are not proteolytically processed, but redistribute across the nuclear envelope independently and prior to caspase activation. Also, mRNA accumulates into the nucleus before caspases become active. Microinjection experiments further revealed that early in apoptosis, the nucleus becomes permeable to dextran molecules of 70 kD molecular weight. Redistribution; of import factors and mRNA, as well as nuclear permeabilisation, occur prior to caspase-mediated nucleoporin cleavage, Our findings suggest that the apoptotic programme includes modifications in the machinery responsible for nucleocytoplasmic transport, which are independent from caspase-mediated degradation of nuclear proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:22:43