Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A metaphyseal defect model of the femur for studies of murine bone healing
Autore:
Uusitalo, H; Rantakokko, J; Ahonen, M; Jamsa, T; Tuukkanen, J; Kahari, VM; Vuorio, E; Aro, HT;
Indirizzi:
Univ Turku, Dept Surg, Skeletal Res Program, FIN-20520 Turku, Finland UnivTurku Turku Finland FIN-20520 Res Program, FIN-20520 Turku, Finland Univ Turku, Dept Med Biochem & Mol Biol, Skeletal Res Program, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 Res Program, FIN-20520 Turku, Finland Univ Turku, Dept Dermatol, Skeletal Res Program, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 Res Program, FIN-20520 Turku, Finland Univ Turku, Ctr Biotechnol, Turku, Finland Univ Turku Turku FinlandUniv Turku, Ctr Biotechnol, Turku, Finland Abo Acad Univ, Turku, Finland Abo Acad Univ Turku FinlandAbo Acad Univ, Turku, Finland Univ Oulu, Dept Anat & Cell Biol, Oulu, Finland Univ Oulu Oulu FinlandUniv Oulu, Dept Anat & Cell Biol, Oulu, Finland
Titolo Testata:
BONE
fascicolo: 4, volume: 28, anno: 2001,
pagine: 423 - 429
SICI:
8756-3282(200104)28:4<423:AMDMOT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTITATIVE COMPUTED-TOMOGRAPHY; MOUSE;
Keywords:
biomechanical testing; bone healing; gene therapy; histomorphometry; peripheral quantitative computed tomography; (pQCT); mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Aro, HT Univ Turku, Dept Surg, Skeletal Res Program, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 ram, FIN-20520 Turku, Finland
Citazione:
H. Uusitalo et al., "A metaphyseal defect model of the femur for studies of murine bone healing", BONE, 28(4), 2001, pp. 423-429

Abstract

A bone defect model was developed in the distal metaphysis of the femur for studies on bone healing in the mouse. The circular defect involving 20% of the bone circumference resulted in a 34% reduction in the bending moment compared to intact bone. The healing process was followed using histomorphometry, peripheral quantitative computed tomography (pQCT), biomechanical testing, and molecular biological analyses. Histologically, healing of the defect was characterized by filling of the medullary cavity with trabecular new bone during the first week of healing, and by closing of the cortical window by 6 weeks, Small areas of periosteal chondrogenesis were frequently observed during defect healing. In pQCT, bone mineral content (BMC) of the defect area approached that of intact control bone already by 3 weeks, reflecting the production of trabecular bone. Similarly, the bending strength and stiffness of the healing femur reached the level of intact control femur already at 3 weeks. Bone formation and remodeling was followed by Northern analyses, which demonstrated elevated mRNA levels for bone components (typeI collagen and osteocalcin), and for osteoclastic enzymes (cathepsin K, matrix metalloproteinase-9, and tartrate-resistant acid phosphatase) throughout the healing period. Finally, the applicability of the defect model for gene therapy experiments was tested using adenovirus-mediated transfer of the LacZ reporter gene. Both histochemistry and mRNA analyses demonstrated that the gene was expressed in the repair tissue with the highest expression during the first week of healing. The present model thus provides a standardized environment for studies on induction and remodeling of trabecular newbone in normal and genetically engineered mice. (Bone 28:423-429; 2001) (C) 2001 by Elsevier Science Inc, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:29:20