Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Gap-junctional communication is required for the maturation process of osteoblastic cells in culture
Autore:
Schiller, PC; Dippolito, G; Balkan, W; Roos, BA; Howard, GA;
Indirizzi:
Univ Miami, Sch Med, VA Med Ctr, GRECC, Miami, FL 33125 USA Univ Miami Miami FL USA 33125 Med, VA Med Ctr, GRECC, Miami, FL 33125 USA Univ Miami, Sch Med, VA Med Ctr, Res Serv, Miami, FL 33125 USA Univ MiamiMiami FL USA 33125 , VA Med Ctr, Res Serv, Miami, FL 33125 USA Univ Miami, Sch Med, Dept Med, Miami, FL 33125 USA Univ Miami Miami FL USA 33125 ami, Sch Med, Dept Med, Miami, FL 33125 USA Univ Miami, Sch Med, Dept Biochem & Mol Biol, Miami, FL 33125 USA Univ Miami Miami FL USA 33125 ept Biochem & Mol Biol, Miami, FL 33125 USA Univ Miami, Sch Med, Dept Neurol, Miami, FL 33125 USA Univ Miami Miami FLUSA 33125 , Sch Med, Dept Neurol, Miami, FL 33125 USA Stein Gerontol Inst, Miami, FL USA Stein Gerontol Inst Miami FL USAStein Gerontol Inst, Miami, FL USA
Titolo Testata:
BONE
fascicolo: 4, volume: 28, anno: 2001,
pagine: 362 - 369
SICI:
8756-3282(200104)28:4<362:GCIRFT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; INTERCELLULAR COMMUNICATION; GLYCYRRHETINIC ACID; HORMONAL-REGULATION; BONE SIALOPROTEIN; MC3T3-E1 CELLS; CONNEXIN43; LINE; RESPONSIVENESS; PROTEINS;
Keywords:
gap junctions; 18-alpha-glycyrrhetinic acid; biomineralization; osteoblasts; differentiation; cell coupling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Schiller, PC Univ Miami, Sch Med, VA Med Ctr, GRECC, 11 GRC,1201 NW 16th St, Miami, FL 33125 USA Univ Miami 11 GRC,1201 NW 16th St Miami FL USA 331253125 USA
Citazione:
P.C. Schiller et al., "Gap-junctional communication is required for the maturation process of osteoblastic cells in culture", BONE, 28(4), 2001, pp. 362-369

Abstract

Osteoblastic cells in long-term culture undergo a phenotypic maturation process leading to extracellular matrix (ECM) production and hone nodule (BN)formation. Cell-to-cell communication via gap junctions (GJC) can be detected between osteoblastic cells within 24 h of plating. We evaluated, in long-term cultures of osteoblastic cells, the effect of inhibiting GJC on the phenotypic: maturation process and the expression of specific genes associated with this process, MC3T3-E1 cells were plated, and, after 24 h (day 0) cells were exposed to 18-alpha -glycyrrhetinic acid (AGA), a nontoxic reversible inhibitor of GJC, GJC, alkaline phosphatase (AP) activity, BN formation, and the relative level of transcripts encoding osteocalcin (OC), bone sialoprotein (bSP), osteopontin (OP). collagen alpha1 type I(alpha 1ICol), and elongation factor-1a (EF1a) were evaluated on day 0 and every 4-7 days thereafter through day 30, GJC was assessed by fluorescent dye transfer. Gene expression was analyzed by northern blot and semiquantitative reverse transcription-polymerase chain reaction. GJC was detectable at day 0 and increased with time in culture, AGA (100 mu mol/L) strongly inhibited GJC at alltimepoints tested. Moreover, AGA-exposed tells showed a dose-dependent decrease in AP activity and a delay in the appearance of BN. This delayed phenotypic expression coincided with an inhibitory effect on the expression of the osteoblast-specific genes OC and bSP, Expression of alpha 1ICol mRNA was also affected, but to a lesser extent, whereas OP and EF1a were not affected. Similar results were obtained with oleamide, an additional reversible inhibitor of GJC, In contrast, cells exposed to either vehicle or 100 mu mol/L glycyrrhizic acid (a noninhibitory glycoside of 18-beta -glycyrrhetinicacid) were indistinguishable from untreated cells for ail parameters evaluated. We conclude that GJC inhibition interferes with the maturation process of osteoblastic cells in culture, possibly by affecting signals regulating the expression of genes involved in the maturation/differentiation of theosteoblastic phenotype, (Bone 28:362-369; 2001) (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 08:57:26